Abstract: A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.
Type:
Grant
Filed:
June 17, 1991
Date of Patent:
June 15, 1993
Assignee:
Agri-Diagnostics Associates
Inventors:
Richard K. Lankow, Sally A. Miller, G. David Grothaus, Frank P. Petersen, Dennis R. Stocker, Stephanie L. Papa, James Donovan, Douglas Malik
Abstract: This invention provides monoclonal antibodies useful for the detection of Sclerotinia infection of plants. Hybridoma producing the antibodies as well as materials and kits for carrying out the detection of the organisms are also disclosed.
Type:
Grant
Filed:
December 20, 1988
Date of Patent:
February 16, 1993
Assignee:
Agri-Diagnostics Associates
Inventors:
Frank P. Petersen, Adrianna Maybroda, Sally A. Miller
Abstract: A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.
Type:
Grant
Filed:
May 20, 1986
Date of Patent:
September 10, 1991
Assignee:
Agri-Diagnostics Associates
Inventors:
Richard K. Lankow, Sally A. Miller, G. David Grothaus, Frank P. Petersen, Dennis R. Stocker, Stephanie L. Papa, James Donovan, Douglas Malik
Abstract: This invention provides monoclonal antibodies useful for the detection of Rhizoctonia infection of plants. Hybridoma producing the antibodies as well as materials and kits for carrying out the detection of the organisms are also disclosed.
Type:
Grant
Filed:
April 28, 1987
Date of Patent:
November 7, 1989
Assignee:
Agri-Diagnostics Associates
Inventors:
Frank P. Petersen, Gary D. Grothaus, Sally A. Miller
Abstract: This invention provides monoclonal antibodies useful for the detection of Phythiaceae infection of plants. Hybridoma producing the antibodies as well as materials and kits for carrying out the detection of the organisms are also disclosed.
Type:
Grant
Filed:
September 9, 1985
Date of Patent:
July 4, 1989
Assignee:
Agri-Diagnostics Associates
Inventors:
Frank P. Petersen, Adrianna M. Maybroda, Gary D. Grothaus, Sally A. Miller
Abstract: This invention provides monoclonal antibodies which specifically bind to Sclerotinia homoeocarpa the causative agent of dollarspot disease in plants. Hybridoma producing the antibodies as well as materials and kits for carrying out the detection of Sclerotina homoeocarpa are also disclosed.
Type:
Grant
Filed:
February 27, 1986
Date of Patent:
February 7, 1989
Assignee:
Agri-Diagnostics Associates
Inventors:
Frank P. Petersen, Adrianna M. Maybroda, Sally A. Miller