Abstract: Disclosed is a self-incompatible dihaploid potato and a production method therefor, and a hybrid potato and a seed production method therefor. The production method includes the following steps: haploid-inducing a diploid potato carrying one or more heterozygous self-compatibility genes by a haploid induction line, and screening haploid mutants; selecting a haploid mutant which does not contain any self-compatible gene; and performing chromosome doubling on the haploid mutant which does not contain any self-compatibility gene to obtain a self-incompatible dihaploid potato line. The self-incompatible dihaploid potato is used as a female parent for seed production, such that emasculation may be avoided, hybrids do not bear fruits, and the yield of the potato is increased.
Type:
Application
Filed:
September 15, 2024
Publication date:
January 2, 2025
Applicants:
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences ( Shenzhen, Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences
Abstract: Provided is primers, where the primers specifically bind to a polynucleotide and are used to amplify the polynucleotide; and the polynucleotide comprises or consists of the following sequence: 1) the nucleotide sequence represented by SEQ ID NO: 5; or 2) the complete complementary sequence of the sequence represented by SEQ ID NO: 5; or 3) a nucleotide sequence being identical to 300 bp or more contiguous nucleotides in the sequence of the above 1) or 2), where the nucleotide sequence being identical to 300 bp or more contiguous nucleotides is unique to a male Ginkgo biloba plant; and the sequences of the primers are respectively represented by SEQ ID NO: 1 and SEQ ID NO: 2.
Type:
Grant
Filed:
December 16, 2021
Date of Patent:
June 4, 2024
Assignees:
Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, AGRICULTURAL GENOMICS INSTITUTE AT SHENZHEN, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Inventors:
Jianbin Yan, Qinggang Liao, Junbo Gou, Ran Du, Sanwen Huang
Abstract: Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Ss11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.
Type:
Grant
Filed:
July 7, 2020
Date of Patent:
September 5, 2023
Assignees:
Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, AGRICULTURAL GENOMICS INSTITUTE AT SHENZHEN, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Inventors:
Sanwen Huang, Chunzhi Zhang, Zhongmin Yang, Die Tang