Abstract: The invention provides a liquid sampling device and method using a chamber in which a vacuum is created to draw a liquid sample through a sample intake tube assembly into a sample receptacle placed in the chamber. The vacuum is created by a vacuum pump attached to an entry port in the chamber. The sample intake tube assembly is inserted through a second entry port in the chamber. One end of the sample intake tube assembly is placed in the open sample receptacle located in the chamber, while the other end is placed in the sample site. The independence of the sample intake tube assembly and the vacuum pump stem promotes sample purity. The sample intake tube assembly has a flexible collar which is inserted into and creates a seal with the entry port of the chamber.