Abstract: A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.
Type:
Grant
Filed:
December 21, 2012
Date of Patent:
February 16, 2016
Assignee:
AJ INNUSCREEN, GmbH
Inventors:
Timo Hillebrand, Claus Knippschild, Benjamin Jaschinsky, Elmara Graser
Abstract: A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.
Abstract: A universally usable method for specific detection of target nucleic acid sequences, which method can be performed very rapidly and also simply and furthermore which does not need any expensive instrumental systems. The method is intended to be suitable as a molecular genetic rapid test and to respect the requirements of diagnostic specificity assurance. In this regard it is important that only one specific amplification product be detected and that amplification artifacts can be unambiguously discriminated. A nucleic acid amplification kit suitable for performing this method.
Abstract: Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol.
Abstract: A reaction unit containing a bottom part and a top part for pipetting a liquid, wherein the bottom part contains a reaction cavity with a permeable filter grid insert and the top part contains a reaction cavity which can be fixed on said bottom part and contains a cavity or covering for receiving a magnet.
Type:
Grant
Filed:
May 6, 2008
Date of Patent:
December 3, 2013
Assignees:
AJ Innuscreen GmbH, AJ Cyberton Gesellschaft fuer Laborautomationssysteme Mbh
Inventors:
Timo Hillebrand, Matthias Arndt, Uwe Wellnitz, Klaus Berka, Volker Hillebrand
Abstract: The invention relates to a device, method and test kit for carrying out molecular-biological reactions, wherein the different components for the molecular-biological reactions are located on a solid carrier in different, spatially separated compartments prior to the start of the reaction. The carrier is preferably a porous filter disk made of polyethylene. Fields of application are the amplification of nucleic acids, for example PCR or RealTime PCR, the reverse transcription of RNA in DNA enzyme-substrate interactions or antigen-antibody interactions, or protein synthesis.
Type:
Application
Filed:
July 22, 2011
Publication date:
October 24, 2013
Applicant:
aj Innuscreen GmbH
Inventors:
Timo Hillebrand, Elmara Graser, Katjana Daskalow
Abstract: The invention relates to a device that allows a target nucleic acid to be detected in a homogeneous batch using two different detection formats. The device comprises at least two heatable sample blocks and a reaction cartridge which contains a base (1, 15) and at least one film (3, 4) sealing the base (1, 15), wherein the film (3, 4) comprises a surface (13, 14) that is not connected to the base (1, 15) and said surface (13, 14) forms a volume (16) for media transfer or at least one reaction chamber (10). The device can be used in particular for mobile gene diagnostics under field conditions.
Type:
Application
Filed:
April 8, 2011
Publication date:
June 20, 2013
Applicant:
aj Innuscreen GmbH
Inventors:
Timo Hillebrand, Claus Knippschild, Elmara Graser
Abstract: The present invention relates to a method and test kit for detecting specific nucleic acid sequences, comprising the steps of: 1. matrix-dependent new synthesis of the target nucleic acid; 2. target-specific probe hybridization; and 3. detection of the hybridization event. The invention is characterized in that, in the first step, an oligonucleotide 1, which is marked by a marker 1 and is entirely or partially complementary to the target sequence, acts as a primer in the matrix-dependent new synthesis of the target nucleic acid and, in the second step, an oligonucleotide 2, which is marked by a marker 2 and, owing to its melting temperature being lower than that of the oligonucleotide 1, is not involved in the first step, partially or completely hybridizes with the DNA new synthesis product of oligonucleotide 1.The detection of the hybridization reaction can take place both fluorometrically in the form of a homogeneous assay and, for verification of the result, subsequently immunologically.
Abstract: The invention relates to a universal and greatly simplified method as well as a composition for isolating nucleic acids from different starting materials containing nucleic acids. The composition contains at least one buffer solution for proteolytically solubilizing biological samples, the buffer containing no chaotropic or antichaotropic component, at least one alcoholic component and/or a detergent, a solid phase, and a wash and elution buffer.
Abstract: A simple and convenient method for concentrating a biomolecule, including protein or nucleic acid molecules, from a sample. Purified and isolated biomolecules obtained by this method. Methods for improving the specificity or sensitivity of detecting a biomolecule by concentration and/or purification or isolation of the biomolecule according to the method of the invention.
Abstract: A composition suitable for the isolation of a nucleic acid from a material containing the nucleic acid contains at least one buffer with a chaotropic component; at least one proteolytic enzyme; at least one buffer with an non-chaotropic component; at least one alcoholic component; and a detergent.
Abstract: The invention relates to a mobile device system, comprising a hand-held device and a test kit for the mobile isolation of nucleic acids. The hand-held device comprises at least one sample block for inserting sample containers, a sample block holder with boreholes or recesses for accommodating the sample blocks, a device base with electronic control units for the sample blocks, a voltage source and a connection to the sample block holder, as well as a test kit for the isolation of nucleic acids. The hand-held device is characterized in that the sample blocks can be removed from the sample block holder and the sample block holder can be removed from the device base.
Type:
Application
Filed:
August 16, 2010
Publication date:
April 21, 2011
Applicant:
AJ INNUSCREEN GmbH
Inventors:
Timo HILLEBRAND, Claus KNIPPSCHILD, Benjamin JASCHINSKY
Abstract: A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.
Type:
Application
Filed:
June 22, 2010
Publication date:
February 17, 2011
Applicant:
AJ INNUSCREEN GmbH
Inventors:
Timo Hillebrand, Claus Knippschild, Benjamin Jaschinsky, Elmara Graser
Abstract: A universally usable method for specific detection of target nucleic acid sequences, which method can be performed very rapidly and also simply and furthermore which does not need any expensive instrumental systems. The method is intended to be suitable as a molecular genetic rapid test and to respect the requirements of diagnostic specificity assurance. In this regard it is important that only one specific amplification product be detected and that amplification artifacts can be unambiguously discriminated. A nucleic acid amplification kit suitable for performing this method.
Abstract: Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol.
Abstract: A method and a test kit for rapid detection of specific nucleic acid sequences, especially for detection of mutations or single nucleotide polymorphisms (SNPs), in which the detection reaction takes place in two steps. The first step involves the target-specific amplification reaction, coupled with the probe-hybridization reaction using fluorescence-labeled allele-specific amplification primers. In the second step, the fluorescence is detected by means of commercial fluorescence readers. Genotyping is carried out from the ratio of the end-point fluorescence of the samples and negative controls.
Abstract: Parallel isolation of a double-stranded nucleic acid and a single-stranded nucleic acid is possible from a sample that contains these acids, without separating the acids, by mixing the sample with a lysis buffer having high salt concentration or low salt concentration, or having a proteolytic enzyme. The sample that contains nucleic acid before its lysis, or the sample that has already been lysed or homogenized, is adjusted with a binding buffer in such a manner that the total nucleic acid is adsorbed onto a solid carrier. The binding buffer contains at least one non-ionic detergent in a high concentration. With the exception of the detergent, the sample contains no other non-acidic organic component miscible in water. The carrier with the adsorbed total nucleic acid is removed. The adsorbed total nucleic acid is washed and eluted.
Abstract: A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.
Abstract: Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol.
Abstract: A reaction unit containing a bottom part and a top part for pipetting a liquid, wherein the bottom part contains a reaction cavity with a permeable filter grid insert and the top part contains a reaction cavity which can be fixed on said bottom part and contains a cavity or covering for receiving a magnet.
Type:
Application
Filed:
May 6, 2008
Publication date:
March 12, 2009
Applicants:
AJ INNUSCREEN GMBH, AJ CYBERTRON GESELLSCHAFT FUER LABORAUTOMATIONSSYTEME MBH
Inventors:
Timo Hillebrand, Matthias Arndt, Uwe Wellnitz, Klaus Berka, Volker Hillebrand