Patents Assigned to Akonni Biosystems
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Patent number: 8759085Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.Type: GrantFiled: April 29, 2011Date of Patent: June 24, 2014Assignee: Akonni Biosystems, Inc.Inventors: Phil Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie
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Patent number: 8680026Abstract: A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.Type: GrantFiled: July 21, 2010Date of Patent: March 25, 2014Assignee: Akonni Biosystems, Inc.Inventors: Christopher G. Cooney, David E. Sipes, Rebecca C. Holmberg, Phillip Belgrader
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Patent number: 8680025Abstract: A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.Type: GrantFiled: May 9, 2008Date of Patent: March 25, 2014Assignee: Akonni Biosystems, Inc.Inventor: Christopher G. Cooney
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Patent number: 8623789Abstract: An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system.Type: GrantFiled: September 20, 2010Date of Patent: January 7, 2014Assignee: Akonni Biosystems, Inc.Inventors: Phillip Belgrader, Christopher G. Cooney, Aleksandr N. Perov
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Patent number: 8603783Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.Type: GrantFiled: August 1, 2012Date of Patent: December 10, 2013Assignee: Akonni Biosystems, Inc.Inventors: Phillip Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie
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Patent number: 8574923Abstract: A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge.Type: GrantFiled: November 20, 2012Date of Patent: November 5, 2013Assignee: Akonni Biosystems, Inc.Inventors: Christopher G. Cooney, Phillip Belgrader
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Publication number: 20130078619Abstract: A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge.Type: ApplicationFiled: November 20, 2012Publication date: March 28, 2013Applicant: Akonni Biosystems, Inc.Inventor: Akonni Biosystems, Inc.
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Publication number: 20130079253Abstract: A microarray-based sample analysis (MBSA) system includes a cartridge holder adapted to receive a replaceable cartridge that is configured to receive a detachable, replaceable sample analysis unit containing one or more reaction chambers for sample analysis; a fluid control subsystem that controls fluid flow; and an optical subsystem configured to capture an image of the microarray.Type: ApplicationFiled: July 5, 2012Publication date: March 28, 2013Applicant: AKONNI BIOSYSTEMSInventors: Phillip Belgrader, Christopher G. Cooney, Aleksandr N. Perov
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Patent number: 8399190Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.Type: GrantFiled: September 20, 2010Date of Patent: March 19, 2013Assignees: Akonni Biosystems, Quantilife, Inc.Inventors: Philip Belgrader, Benjamin Hindson
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Patent number: 8334117Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.Type: GrantFiled: April 29, 2011Date of Patent: December 18, 2012Assignee: Akonni Biosystems, Inc.Inventors: Phil Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie
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Patent number: 8329433Abstract: A method for performing a polymerase chain reaction (PCR). The method comprises the step of maintaining a reaction mixture in a PCR reaction chamber alternatively at a first temperature and a second temperature using a device comprising a first heater for heating a temperature-control substance to a first temperature, a second heater for heating said temperature-control substance to a second temperature, a pump located in between and connected in series with the first heater and the second heater; and a bladder unit connected to the first and second heaters via different ports. The pump introduces the temperature-control substance into the bladder unit at the first temperature and the second temperature alternatively with a regular interval to enable the PCR.Type: GrantFiled: April 29, 2011Date of Patent: December 11, 2012Assignee: Akonni Biosystems, Inc.Inventor: Phillip Belgrader
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Publication number: 20120283423Abstract: Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass frit.Type: ApplicationFiled: July 3, 2012Publication date: November 8, 2012Applicant: AKONNI BIOSYSTEMSInventors: Phillip Belgrader, Christopher G. Cooney
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Patent number: 8236501Abstract: Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass fit.Type: GrantFiled: June 3, 2010Date of Patent: August 7, 2012Assignee: Akonni Biosystems, Inc.Inventor: Phillip Belgrader
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Patent number: 8236553Abstract: Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass frit.Type: GrantFiled: June 3, 2010Date of Patent: August 7, 2012Assignee: Akonni Biosystems, Inc.Inventor: Phillip Belgrader
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Publication number: 20120196767Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.Type: ApplicationFiled: April 13, 2012Publication date: August 2, 2012Applicant: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. COONEY, Peter Qiang QU, Alexander PEROV, Jennifer PARKER
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Publication number: 20120149603Abstract: An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.Type: ApplicationFiled: December 8, 2011Publication date: June 14, 2012Applicant: AKONNI BIOSYSTEMSInventors: Christopher G. COONEY, Phillip Belgrader, Arial Bueno, Steve Garber, Nitu Harshendu Thakore, Peter Qiang Qu
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Patent number: 7955841Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.Type: GrantFiled: September 22, 2008Date of Patent: June 7, 2011Assignee: Akonni BiosystemsInventors: Phil Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie
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Patent number: 7955840Abstract: Methods and devices for performing chemical reactions under controlled temperatures are described. In one embodiment, the devices provided by the invention comprise a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing. The reaction chamber has thermally conductive interior and exterior surfaces defining an internal volume therein at a first temperature. The device also includes at least one thermally conductive temperature-control bladder disposed therein, which bladder is configured to receive a temperature-control substance at a second temperature into said bladder and expel said temperature-control substance from said bladder. The bladder is further configured such that upon receiving the temperature-control substance, the bladder expands to abut substantially at least a portion of said exterior surfaces of said reaction chamber to enable thermal exchange between said temperature-control substance the said internal volume of reaction chamber.Type: GrantFiled: August 23, 2007Date of Patent: June 7, 2011Assignee: Akonni BiosystemsInventor: Phil Belgrader
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Publication number: 20100284859Abstract: A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.Type: ApplicationFiled: July 21, 2010Publication date: November 11, 2010Applicant: Akonni BiosystemsInventors: Christopher G. Cooney, David E. Sipes, Rebecca C. Holmberg, Phillip Belgrader
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Publication number: 20100240882Abstract: Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass fit.Type: ApplicationFiled: June 3, 2010Publication date: September 23, 2010Applicant: Akonni BiosystemsInventor: Phillip Belgrader