Abstract: A method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of:
(i) contacting the binding partners, one of which is immobilised on a surface;
(ii) oscillating the surface at increasing amplitude; and
(iii) detecting a dissociation event.
An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface (10) having one binding partner (16) immobilised thereon; means for oscillating the surface at increasing amplitude; and a device (14, 15) for detecting a dissociation event.
Type:
Grant
Filed:
November 15, 2000
Date of Patent:
July 8, 2003
Assignee:
Akubio Ltd.
Inventors:
David Klenerman, Victor Petrovich Ostanin, Fedor Nikolaievich Dultsev