Abstract: The present invention relates to nucleic acid molecules corresponding to regulatory portions of genes whose expression is constitutive. The invention also relates to compositions and methods of using the same to regulate the expression, in a constitutive manner, of genes and/or any kind of nucleotide sequences in a plant. Nucleic acid molecules and its compositions include novel nucleotide sequences for constitutive promoter identified in and isolated from poplar (Populus spp). Methods for expressing genes and/or any kind of nucleotide sequences in a plant using the promoter sequences disclosed herein are provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to one or more of the constitutive promoters of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Abstract: The present invention relates to nucleic acid molecules corresponding to regulatory portions of genes whose expression is constitutive. The invention also relates to compositions and methods of using the same to regulate the expression, in a constitutive manner, of genes and/or any kind of nucleotide sequences in a plant. Nucleic acid molecules and its compositions include novel nucleotide sequences for constitutive promoter identified in and isolated from poplar (Populus spp). Methods for expressing genes and/or any kind of nucleotide sequences in a plant using the promoter sequences disclosed herein are provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to one or more of the constitutive promoters of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Abstract: Disclosed are nucleic acids from the Citrus Leprosis Virus (CiLV) genome, associated with Citrus Leprosis (CiL) disease. Cloned CiLV nucleic acids can be used as probes or primers in assays for detecting CiLV in biological samples, particularly leaves, roots and other tissues of plants, such as plants from Citrus and Poncirus. The nucleic acid molecules can be introduced in cloning vectors and cloning the recombinant nucleic acid molecules in cells, such as prokaryotes, and eukaryotes. The cloned CiLV nucleic acids are expressed in cells to provide immunogenic proteins which can be used to raise antibodies against CiLV, which can then be used to detect CiLV virus in biological samples. Also disclosed are SEQ ID Nos. 5 and 8, in whole or part, transgenic plants, such as monocots and dicots, containing CiLV nucleic acids, in any combination, so that expression increases resistance to CiL disease.
Type:
Grant
Filed:
September 6, 2005
Date of Patent:
May 17, 2011
Assignee:
Alellyx S.A.
Inventors:
Renata Castiglioni Pascon, Ana Claudia Rasera Silva
Abstract: The present invention related to nucleic acid molecules corresponding to regulatory portions of genes whose expression is predominant in cambium and/or xylem. The invention also relates to compositions and methods of using the same to regulate the expression, in a cambium/xylem-preferred manner, of genes and/or any kind of nucleotide sequences in a plant. Nucleic acid molecules and its compositions include novel nucleotide sequences for cambium/xylem-preferred promoters identified and isolated from poplar (Populus spp). Methods for expressing genes and/or any kind of nucleotide sequences in a plant using the promoter sequences disclosed herein are provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to a cambium/xylem-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Type:
Application
Filed:
June 13, 2007
Publication date:
September 10, 2009
Applicant:
ALELLYX S.A.
Inventors:
Fabio Papes, Isabel Gerhardt, Paulo Arruda