Abstract: Systems and methods for sterilizing liquid media such as saline water using filtration and ozonation.

Abstract: One embodiment of the invention provides a genetically enhanced Chlorogloeopsis sp. host cell comprising at least one first recombinant gene encoding a first protein for the production of ethanol under the transcriptional control of a first inducible promoter, having at least 85%, 90% or 95% sequence identity to an endogenous inducible promoter of the Chlorogloeopsis sp. host cell.

Abstract: Methods of inoculum scale-up of cyanobacteria to commercial-scale closed photobioreactors for the production of a target molecule such as ethanol.

Abstract: A plasmid vector for the production of compounds in cyanobacteria is described which is capable of being efficiently transformed to and replicating in a broad range of cyanobacterial species.

Abstract: Disclosed herein are processes for removing water from organic solvents, such as ethanol. The processes include distillation in two columns operated at sequentially higher pressure, followed by treatment of the overhead vapor by one or two membrane separation steps.

Abstract: A cyanobacterium for the production of isoprene having an extrachromosomal plasmid harboring a gene for the production of isoprene. Such a cyanobacterium exhibits a higher isoprene production rate than other conventional strains.

Abstract: Cyanobacterial host cells are modified to produce useful chemicals such as 1,3-propanediol and glycerol.

Abstract: Methods are provided for analyses of restriction endonucleases.

Abstract: Silicalite particles, methods of making silicalite particles, systems comprising silicalite particles and methods for using silicalite particles to separate ethanol from water.

Abstract: A liquid agitation system comprising an agitation element designed to agitate and mix a liquid medium when in motion on the surface of the liquid medium, thereby enhancing energy efficiency of mass transfer into and out of the liquid medium, a kit for the liquid agitation system and a method of using the agitation element.

Abstract: A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

Abstract: One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

Abstract: This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds.

Abstract: A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

Abstract: A method of rapid simultaneous inoculation of cyanobacteria to multiple commercial-scale closed photobioreactors for the production of a target molecule such as ethanol.

Abstract: A process is described for the creation of an inoculum for an organism capable of photosynthesis of a target compound within a closed bioreactor, involving steps of transfer of a sterile growth medium under pressure without exposure to atmosphere. The process is useful for the creation of inocula for the growth of cyanobacteria to produce target chemical products, such as ethanol, in closed photobioreactors.

Abstract: Methods and compositions are provided for the full segregation of recombinant marine cyanobacteria.

Abstract: A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

Abstract: A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.

Abstract: One embodiment of the invention provides a genetically enhanced cyanobacterium producing a first chemical compound comprising: a genetically enhanced genome with a first gene inactivation in a first essential or conditionally essential gene of the cyanobacterium, and a first extrachromosomal plasmid harboring the first essential or conditionally essential gene and at least one first production gene for production of the first chemical compound, wherein the cyanobacterium lacks a functional gene conferring biocide resistance. These cyanobacteria can produce a first valuable chemical compound in long-term cultures without the need to employ genes conferring biocide resistance.