Abstract: The structure of the T. reesei xln1 and xln2 genes and the primaru structure of proteins are described. Enzyme preparations enriched in hemicellulase enzymes are described. Such enzyme preparations may also be partially or completely deficient in cellulose degrading activity. Such preparations may be utilized in an crude, unpurified form and are especially useful in the production of pulp and paper.
Type:
Grant
Filed:
September 16, 1993
Date of Patent:
November 17, 1998
Assignee:
Alko-Yhtiot Oy
Inventors:
Pirkko Suominen, Helena Nevalainen, Ritva Saarelainen, Marja Paloheimo, Richard Fagerstrom
Abstract: Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.
Type:
Grant
Filed:
June 23, 1994
Date of Patent:
June 25, 1996
Assignee:
Alko-Yhtiot Oy
Inventors:
Jonathan Knowles, Merja Penttila, Tuula Teeri, Helena Nevalainen, Paivi Lehtovaara-Helenius
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