Abstract: The invention provides oxygen- resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H2 in a light catalyzed reaction having water as the reactant.
Type:
Grant
Filed:
April 16, 2004
Date of Patent:
March 10, 2009
Assignee:
Allaince for Sustainable Energy, LLC
Inventors:
Paul King, Maria L Ghirardi, Michael Seibert