Abstract: This invention generally relates to apparatus and methods for high-throughput, reproducible and inexpensive detection of virus infection using multiplexing technologies such as slide-based, microtiter plate-based and membrane-based microarrays and beads technologies. The apparatus and methods allow simultaneous detection of multiple viral infections in a plurality of test samples.
Type:
Grant
Filed:
April 12, 2005
Date of Patent:
May 25, 2010
Assignee:
Allied Biotech, Inc.
Inventors:
Yongyi Yu, Lina Li, Zhuangwu Li, Jun Bao
Abstract: A method for determining telomerase activity using primer extension followed with real time PCR quantification is disclosed. The method of the present invention provides a rapid, sensitive and accurate measurement for telomerase activity in a biological sample. In one embodiment, the method includes the steps of: adding the biological sample to a reaction tube containing a first reaction mixture having a first primer and nucleoside triphosphates, a second reaction mixture having a second primer and a DNA polymerase, and a wax layer that separates the first reaction mixture from the second reaction mixture; incubating the biological sample with the first reaction mixture; admixing the extension product with the second reaction mixture; amplifying and quantifying the extension product using real-time PCR and a control template. In another embodiment, the detection method includes an in situ primer extension step that allows the production of the extension product within an intact cell.
Type:
Grant
Filed:
November 12, 2003
Date of Patent:
June 24, 2008
Assignee:
Allied Biotech, Inc.
Inventors:
Zhunagwu Li, Jun Bao, Hua Mao, Wenbin Ma, Lina Li
Abstract: A method for determining telomerase activity using primer extension followed with real time PCR quantification is disclosed. The method of the present invention provides a rapid, sensitive and accurate measurement for telomerase activity in a biological sample. In one embodiment, the method includes the steps of: adding the biological sample to a reaction tube containing a first reaction mixture having a first primer and nucleoside triphosphates, a second reaction mixture having a second primer and a DNA polymerase, and a wax layer that separates the first reaction mixture from the second reaction mixture; incubating the biological sample with the first reaction mixture; admixing the extension product with the second reaction mixture; amplifying and quantifying the extension product using real-time PCR and a control template. In another embodiment, the detection method includes an in situ primer extension step that allows the production of the extension product within an intact cell.
Type:
Application
Filed:
November 12, 2003
Publication date:
November 2, 2006
Applicant:
Allied Biotech, Inc.
Inventors:
Li Zhuangwu, Bao Jun, Ma Wenbin, Lina Li