Patents Assigned to ALTRATECH LIMITED
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Patent number: 12071616Abstract: A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.Type: GrantFiled: March 5, 2021Date of Patent: August 27, 2024Assignees: Altratech Limited, The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Brian O'Farrell, Claire O'Connell, Kaylyn Oshaben, Daniel Appella
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Patent number: 11796498Abstract: An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.Type: GrantFiled: June 30, 2020Date of Patent: October 24, 2023Assignee: Altratech LimitedInventors: Timothy Cummins, Brian O'Farrell
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Patent number: 11459601Abstract: A portable diagnostic device has a lysate stage with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages are linked with the lysate vessel, each with a reservoir linked by channels. The final stage has a sensor for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.Type: GrantFiled: September 6, 2018Date of Patent: October 4, 2022Assignee: Altratech LimitedInventors: Brian O'Farrell, Cian Desmond O'Sullivan, John O'Driscoll, Timothy Cummins, Paul Free, Moira Mccarthy, John Walshe
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Patent number: 11274291Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel, within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads which are initially embedded in a wax body and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket along with the wax, which is allowed to solidify before they are removed from the reaction vessel.Type: GrantFiled: November 20, 2018Date of Patent: March 15, 2022Assignee: Altratech LimitedInventors: Brian O'Farrell, Timothy Cummins, Cian Desmond O'Sullivan, Jorge Alvarez-Vicente
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Patent number: 10995331Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel, within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads which are initially embedded in a wax body and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket along with the wax, which is allowed to solidify before they are removed from the reaction vessel.Type: GrantFiled: November 20, 2018Date of Patent: May 4, 2021Assignee: Altratech LimitedInventors: Brian O'Farrell, Timothy Cummins, Cian Desmond O'Sullivan, Jorge Álvarez-Vicente
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Patent number: 10746683Abstract: An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.Type: GrantFiled: December 10, 2014Date of Patent: August 18, 2020Assignee: ALTRATECH LIMITEDInventors: Timothy Cummins, Brian O'Farrell
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Patent number: 10738348Abstract: Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or “tethers” the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.Type: GrantFiled: December 10, 2014Date of Patent: August 11, 2020Assignee: ALTRATECH LIMITEDInventors: Brian O'Farrell, Timothy Cummins
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Publication number: 20200229742Abstract: A diagnostic sensor device has a semiconductor chip having a distal end physically configured to fit into a power and data socket conforming to a non-proprietary standard, and having exposed pads for engagement with corresponding conductors of such a socket. At its proximal end the chip has at least one sensor for contact with an analyte. The device may be manufactured in a single integrated process to provide a wafer which is diced to provide the individual devices.Type: ApplicationFiled: September 6, 2018Publication date: July 23, 2020Applicants: UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK, ALTRATECH LIMITEDInventors: Brian O'FARRELL, Ivan O'CONNELL, Steven DARBY, Timothy CUMMINS
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Publication number: 20200216884Abstract: A portable diagnostic device has a lysate stage with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages are linked with the lysate vessel, each with a reservoir linked by channels. The final stage has a sensor for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.Type: ApplicationFiled: September 6, 2018Publication date: July 9, 2020Applicant: ALTRATECH LIMITEDInventors: Brian O'FARRELL, Cian Desmond O'SULLIVAN, John O'DRISCOLL, Timothy CUMMINS, Paul FREE, Moira MCCARTHY, John WALSHE
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Publication number: 20190085319Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel, within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads which are initially embedded in a wax body and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket along with the wax, which is allowed to solidify before they are removed from the reaction vessel.Type: ApplicationFiled: November 20, 2018Publication date: March 21, 2019Applicant: ALTRATECH LIMITEDInventors: Brian O'FARRELL, Timothy CUMMINS, Cian Desmond O'SULLIVAN, Jorge ÁLVAREZ-VICENTE
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Publication number: 20190085320Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel, within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads which are initially embedded in a wax body and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket along with the wax, which is allowed to solidify before they are removed from the reaction vessel.Type: ApplicationFiled: November 20, 2018Publication date: March 21, 2019Applicant: ALTRATECH LIMITEDInventors: Brian O'FARRELL, Timothy CUMMINS, Cian Desmond O'SULLIVAN, Jorge ÁLVAREZ-VICENTE
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Patent number: 10160966Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel (11), within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads (26) which are initially embedded in a wax body (17) and are released during the heating so that they are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket (16) along with the wax (17), which is allowed to solidify before they are removed from the reaction vessel.Type: GrantFiled: December 10, 2014Date of Patent: December 25, 2018Assignee: ALTRATECH LIMITEDInventors: Brian O'Farrell, Timothy Cummins, Cian Desmond O'Sullivan, Jorge Álvarez-Vicente
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Publication number: 20170023512Abstract: An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.Type: ApplicationFiled: December 10, 2014Publication date: January 26, 2017Applicant: ALTRATECH LIMITEDInventors: Timothy Cummins, Brian O'Farrell
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Publication number: 20160304941Abstract: Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or “tethers” the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.Type: ApplicationFiled: December 10, 2014Publication date: October 20, 2016Applicant: ALTRATECH LIMITEDInventors: Brian O'FARRELL, Timothy CUMMINS
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Publication number: 20160298107Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel (11), within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads (26) which are initially embedded in a wax body (17) and are released during the heating so that they are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket (16) along with the wax (17), which is allowed to solidify before they are removed from the reaction vessel.Type: ApplicationFiled: December 10, 2014Publication date: October 13, 2016Applicant: ALTRATECH LIMITEDInventors: Brian O'FARRELL, Timothy CUMMINS, Cian Desmond O'SULLIVAN, Jorge ÁLVAREZ-VICENTE