Patents Assigned to Ambergen
-
Publication number: 20240159749Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.Type: ApplicationFiled: November 28, 2023Publication date: May 16, 2024Applicants: AMBERGEN, INC., THE GENERAL HOSPITAL CORPORATIONInventors: MARK J. LIM, HEATHER P. OSTENDORFF, KENNETH J. ROTHSCHILD, DONALD B. BLOCH
-
Patent number: 11940447Abstract: The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands.Type: GrantFiled: July 12, 2022Date of Patent: March 26, 2024Assignee: AMBERGEN, INC.Inventors: Mark J. Lim, Gargey Yagnik, Kenneth J. Rothschild
-
Patent number: 11906527Abstract: The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands.Type: GrantFiled: August 11, 2021Date of Patent: February 20, 2024Assignee: AMBERGEN, INC.Inventors: Mark J. Lim, Gargey Yagnik, Kenneth J. Rothschild
-
Patent number: 11885802Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.Type: GrantFiled: March 24, 2017Date of Patent: January 30, 2024Assignees: AMBERGEN, INC., MASSACHUSETTS GENERAL HOSPITALInventors: Mark J. Lim, Heather P. Ostendorff, Kenneth J. Rothschild, Donald B. Bloch
-
Patent number: 11846634Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.Type: GrantFiled: April 3, 2020Date of Patent: December 19, 2023Assignee: AMBERGEN, INC.Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
-
Patent number: 11789027Abstract: The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. The field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout, and also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI.Type: GrantFiled: August 11, 2021Date of Patent: October 17, 2023Assignee: AmberGen, Inc.Inventors: Mark J. Lim, Gargey Yagnik, Kenneth J. Rothschild
-
Patent number: 11782056Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.Type: GrantFiled: April 3, 2020Date of Patent: October 10, 2023Assignee: AmberGen, Inc.Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
-
Patent number: 10088474Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.Type: GrantFiled: October 6, 2015Date of Patent: October 2, 2018Assignee: AmberGen, Inc.Inventors: Mark J Lim, Kenneth J Rothschild
-
Patent number: 10060912Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.Type: GrantFiled: October 3, 2016Date of Patent: August 28, 2018Assignee: AmberGen, Inc.Inventors: Mark J Lim, Vladislav B Bergo, Kenneth J Rothschild
-
Patent number: 9910034Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.Type: GrantFiled: January 14, 2015Date of Patent: March 6, 2018Assignee: AmberGen, Inc.Inventors: Mark J. Lim, Kenneth J. Rothschild
-
Patent number: 9523680Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.Type: GrantFiled: June 29, 2011Date of Patent: December 20, 2016Assignee: AmberGen, Inc.Inventors: Mark J. Lim, Kenneth J. Rothschild
-
Patent number: 9513285Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.Type: GrantFiled: December 17, 2013Date of Patent: December 6, 2016Assignee: AmberGen, Inc.Inventors: Mark J. Lim, Vladislav B. Bergo, Kenneth J. Rothschild
-
Patent number: 9334530Abstract: Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).Type: GrantFiled: May 13, 2010Date of Patent: May 10, 2016Assignee: AmberGen, Inc.Inventors: Mark J. Lim, Kenneth J. Rothschild
-
Patent number: 8932879Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.Type: GrantFiled: October 30, 2008Date of Patent: January 13, 2015Assignee: Ambergen, Inc.Inventors: Mark J. Lim, Kenneth J. Rothschild
-
Patent number: 8906700Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.Type: GrantFiled: July 19, 2012Date of Patent: December 9, 2014Assignee: Ambergen, Inc.Inventors: Mark J. Lim, Kenneth J. Rothschild
-
Patent number: 8852956Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.Type: GrantFiled: October 5, 2010Date of Patent: October 7, 2014Assignees: Ambergen, Inc., Massachusetts General HospitalInventors: Mark J. Lim, Heather P. Ostendorf, Kenneth J. Rothschild, Donald B. Bloch
-
Publication number: 20140235471Abstract: Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.Type: ApplicationFiled: December 17, 2013Publication date: August 21, 2014Applicant: Ambergen, Inc.Inventors: Vladislav B. Bergo, Mark J. Lim, Kenneth J. Rothschild
-
Publication number: 20140081007Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.Type: ApplicationFiled: August 22, 2013Publication date: March 20, 2014Applicant: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
-
Patent number: 8507648Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as mass difference.Type: GrantFiled: August 4, 2010Date of Patent: August 13, 2013Assignee: Ambergen, Inc.Inventors: Alex Garvin, Sadanand Gite, Vladislav B. Bergo, Kenneth J. Rothschild
-
Patent number: 8486634Abstract: Methods of amplifying nucleic acid are described. Primers on solid support, e.g. a population of beads, are employed. A population of nucleic acid template molecules, wherein the nucleic acid template molecules have been treated with bisulfite, is amplified so as to create loaded beads comprising amplified nucleic acid.Type: GrantFiled: November 6, 2008Date of Patent: July 16, 2013Assignee: Ambergen, Inc.Inventors: Mark J. Lim, Kenneth J. Rothschild