Abstract: A device for protein analysis or mapping, includes an array of particulate nanoparticles, wherein said nanoparticles are bound, preferably co-immobilized, at specific spots on a planar read-out surface or substrate and are provided with a plurality of capture probes for capturing proteins, preferably glycoproteins. A method in which the device is used for protein profiling, especially glycoprotein profiling, of individual samples with high sensitivity is also disclosed. The device and method do not require any complex sample preparation.

Abstract: The invention provides contrast agents for optical imaging of lung cancer in patients. The contrast agents may be used in diagnosis of lung cancer, for follow up of progress in disease development, for follow up of treatment of lung cancer and for surgical guidance. Further, the invention provides methods for optical imaging of lung cancer in patients.

Abstract: The present invention relates to devices and method for melting solid polarised sample while retaining a high level of polarisation. In an embodiment of the present invention a sample is polarised in a sample-retaining cup 9 in a strong magnetic field in a polarising means 3a, 3b, 3c in a cryostat 2 and then melted inside the cryostat 2 by melting means such as a laser 8 connected by an optical fibre 4 to the interior of the cryostat.

Abstract: An analytical process is disclosed, for discriminating data acquired from samples with overlapping distributions, and for improving and assessing the statistical validity of hybridization signal in arrays of assays. The process includes method of convolving data into two or more discrete probability density functions representing signal and nonsignal, discrete fluors, or other convolved independent variables. The system uses the probability density functions to assign hybridization signals, objectively, to one of the modeled distributions. Subsequent processes assess variability inherent to the arrays, and use this assessed variation to establish reliability scores and confidence limits for complete hybridization arrays, and for discrete hybridization assays within arrays.

Abstract: A filtration housing for a filter cassette includes a base frame means supporting a first and second parallel plate to be movable thereon relative to each other in directions normal thereto. The filtration cassette being retained between opposing first major surfaces of the parallel plates with the direction of movement thereof aligned with the axis of the membrane sheets. The first plate defines a first channel substantially parallel to and axially aligned with first edges of the cassette, a second channel substantially parallel to and axially aligned with the second edges of the cassette, and a third channel means including a first portion acutely oriented with respect to either of the first and second edges of the cassette and a second portion optionally substantially transversely oriented with respect to the first and second edges of the cassette or acutely oriented thereto and extending a distance therebetween.

Abstract: A method of magnetic resonance (MR) imaging of blood perfusion in vascularized organs of interest, e.g. the myocardium, the kidneys, the brain, the liver wherein a contrast agent composition comprising a particulate superparamagnetic metal oxide is administered.

Abstract: This application relates to the use of certain amines of formula (I) for combatting and preventing systemic and local complement activation, and in particular to the use of meglumine and derivatives and optionally salts thereof in combatting and preventing said activation. The amines are also of use in combatting activation of the kinin/kallikrein system or blood coagulation system.

Abstract: The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

Abstract: Synthetic analogues of lysine and glutamine are provided which function as substrates for the fibrin-stabilising enzyme Factor XIIIa even when labelled with a detectable moiety. The use of suitable protecting groups provides compounds which possess reduced susceptibility to in vivo metabolism especially by peptidases, and are hence useful agents for the diagnosis of thrombosis, embolism, atherosclerosis, inflammation or cancer.

Abstract: The present invention relates to a chip quartz oscillator. In an embodiment of a chip quartz oscillator S in accordance with the present invention, a quartz oscillator 2, which has a detection electrode 3 on a surface thereof and a non-detection electrode on the other surface thereof, is fixed on a substrate 1, and each of the electrodes is connected electrically to a terminal 4 or 4? on the substrate 1. While keeping the non-detection electrode in contact with the substrate 1, the quartz oscillator 2 is fixed on the substrate 1, by flexibly bonding the side-wall 2?? of the quartz oscillator 2 along its circumference to the surface of the substrate 1 by using the elastic bonding agent 5. Thus, the oscillation surface of the quartz oscillator 2 is supported distributively on the substrate 1 in a surface-contacting and non-adhesive manner.

Abstract: The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, characterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have colorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer.

Abstract: A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3??5? exonuclease activity which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on the detection.

Abstract: Disclosed is a compound of Formula (I), wherein: L is a linking agent; B is a binding agent; X is an atom or group suitable for attaching L to the glycerol chain; and R is a straight chain saturated or unsaturated alkyl group having from 8 to 30 carbon atoms, substituted with M? or M? wherein at least one of M? and/or M? is a detectable label. The compound can be used as a lipase substrate in a solid phase-based assay system, such as a scintillation proximity assay, to detect lipase enzyme activity.

Abstract: The present invention relates to the use of particulate materials as contrast agents in in vivo light imaging.

Abstract: A separation material characterized by supporting, in its surface, a linear or crosslinked stimulus responsive polymer to which a substance A having specific affinity for a target substance and a low-molecular substance B having specific affinity for the substance A have been bonded. Such a separation material can increase the recovery of a target substance to be purified and do not require desalting and hapten sugar-eliminating operations and, in addition, have high productivity.

Abstract: Method for the removal of a substance from an aqueous liquid by ion exchange comprising providing a liquid with said substance is present; providing an adsorption matrix which comprises at least two different ligands; contacting the liquid with the matrix under a period of time and conditions sufficient to allow adsorption of the substance to the matrix; and adding an eluent that desorbs the substance from the matrix. Each one of the ligands interacts with the substance during the adsorption step, and at least one of the ligands is charged and capable of ionic interaction with the substance. The method can be run as a cation or anion exchange. The substance desorption can be performed by adding an eluent comprising an increasing ionic strength. The invention also encompasses an adsorbent comprising at least two structurally different ligands, which interact with the same kind of substance when used in a separation procedure.

Abstract: The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatized scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

Abstract: The performance of devices for the detection of fluorophore labelled substances in an electrophoretic separation medium is improved by rescanning lines or pixels which caused saturation of an electromagnetic radiation detecting means (19) at a lower gain, the lower gain being adapted to prevent saturation of the electromagnetic radiation detecting means (19).

Abstract: An instrument treatment station includes a station body having a first and second elongate and spaced channel walls. The channel walls define an elongate instrument channel therebetween. The station body defines an elongate drain channel in fluid communication with the instrument channel. The station body further defines a source port in fluid communication with the instrument channel for delivering a fluid flow into the instrument channel for treating an instrument therein. The instrument treatment station may accommodate arrays of aligned instruments.

Abstract: This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink™, eSensor™, multiplex arrays with cartridges etc., all described herein.