Abstract: A device for protein analysis or mapping, includes an array of particulate nanoparticles, wherein said nanoparticles are bound, preferably co-immobilized, at specific spots on a planar read-out surface or substrate and are provided with a plurality of capture probes for capturing proteins, preferably glycoproteins. A method in which the device is used for protein profiling, especially glycoprotein profiling, of individual samples with high sensitivity is also disclosed. The device and method do not require any complex sample preparation.

Abstract: Method for the removal of a substance from an aqueous liquid by ion exchange comprising providing a liquid with said substance is present; providing an adsorption matrix which comprises at least two different ligands; contacting the liquid with the matrix under a period of time and conditions sufficient to allow adsorption of the substance to the matrix; and adding an eluent that desorbs the substance from the matrix. Each one of the ligands interacts with the substance during the adsorption step, and at least one of the ligands is charged and capable of ionic interaction with the substance. The method can be run as a cation or anion exchange. The substance desorption can be performed by adding an eluent comprising an increasing ionic strength. The invention also encompasses an adsorbent comprising at least two structurally different ligands, which interact with the same kind of substance when used in a separation procedure.

Abstract: The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatized scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

Abstract: This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink™, eSensor™, multiplex arrays with cartridges etc., all described herein.

Abstract: A method for the manufacture of a solid porous separation material based on a polysaccharide, said method comprising the steps of: (a) providing an aqueous solution (I) of a polysaccharide, (b) solidifying the solution, preferably by transforming the solution to a gel, and (c) optionally crosslinking the polysaccharide, with the proviso that, if step (c) is present, steps (b) and (c) may be carried out simultaneously.

Abstract: The present invention relates to valves with small dead volumes. A valve in accordance with the present invention has a ferrule (7) which receives a plurality of fluid lines (33) which lead to outlets on an end surface (25) of said ferrule (7) and a movable selector body (15) which has a fluid groove (37, 37A) on a surface (23) which is in contact with the end surface (25) of said ferrule (7). Means are provided for exerting a clamping force on said ferrule (7) in order to simultaneously clamp all the fluid lines (33).

Abstract: A liquid transfer system (100) comprising a net (170) and a net support (160), wherein, the net (170) is integrally joined with the net support (160). The net (170) and the net support (160) are preferably joined by welding, such as contact welding or by welding at a lower edge (400) of a number of welding-holes (350) that extends through the net support (160).

Abstract: Methods and devices for detecting nucleic acid and protein targets on hydrogel microarrays are disclosed. Fluorophores are incorporated into the targets and detected. A linear correlation between target concentration and signal amplitude is maintained through the elimination of active enzyme amplification.

Abstract: The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.

Abstract: A uniform fluid distribution system (2) for use with a liquid transfer system (100) for maintaining an interface between liquid phases within a large scale separator system including a cell into which liquid may be introduced as discrete phases at an inlet zone occupying a first approximately transverse cross-sectional region of said cell and outputted at an outlet zone occupying a second approximately transverse cross-sectional region of said cell. Said distribution system comprises at least one liquid inlet (24) and at least two distribution outlets (32), which are connected by an internal flow connection system (36).

Abstract: Method for desalting an aqueous liquid containing a charged substance comprising the steps of: (i) contacting liquid (I) with an ion-exchanger (1) under conditions permitting binding between ion-exchanger and substance, said ion-exchanger comprising a base matrix carrying an ion-exchange ligand (Ligand 1) with the opposite charge compared to the substance, (ii) desorbing said substance from said ion-exchanger by the use of a liquid (liquid (II)). Wherein: (A) ion-exchanger (1) is an ion-exchanger: (a) that can bind the substance in an aqueous reference liquid at ionic strength corresponding to 0.1 M NaCl, preferably 0.25 M NaCl; and (b) permits a breakthrough capacity at the pH provided by liquid (I) which is more than 2 mg/ml of gel, at a breakthrough of 10% and linear flow velocity of 300 cm/h; and (B) in step (ii) the pH of liquid (11) is adjusted to a pH value where the charge difference between the substance, the ligand and/or the ion-exchanger is lowered.

Abstract: Method and device for continuously producing a coherent layer of a liquid/melt of even thickness by means of centrifugal action along the entire periphery of a rotating disk (13, 113), liquid/melt being supplied to the disk around its entire axis of rotation from means (12, 112) and leaving the disk as individual droplets. The means (12, 112) distributing the liquid/melt onto the disk (13, 113) has according to the invention essentially the same speed and direction of rotation as the disk, and the liquid/melt undergoes under the influence of centrifugal action circumferentially a spreading and change in velocity across the disk (13, 113) in contact with the same.

Abstract: The invention refers to a method of controlling the porosity of porous spherical particles produced from a polysaccharide dissolved in a solvent, in which it can be gelled. The polysaccharide solution is finely divided by mechanical means into spherical droplets which are allowed to pass through a humid atmosphere and transferred to a capturing medium while controlling the temperature and humidity of humid atmosphere.

Abstract: A method for producing IgG from plasma for medical applications, comprising at least: (i′) removal of albumin resulting in an IgG fraction, (ii′) purifying IgG from an IgG fraction, which is derived from the IgG fraction obtained in step (i′), by adsorbing IgG to a cation exchanger and collecting the adsorbed IgG fraction, and (iii′) virus inactivation in an IgG fraction derived from the IgG fraction collected in step (ii′). The method is characterized in; (I) concentrating the IgG fraction obtained in step (i′), (II) adjusting pH to 4±0.1 in the IgG fraction released from the cation exchanger in step (ii′), and preferably maintaining the pH below 6.0 during the remaining steps of the method; and (III) carrying out the virus inactivation (step iii′) by using chemicals at a temperature of 30° C.±2° C. for at least 4 hours. Anticomplementary activity is typically below 1 CH50/mg immunoglobulin.

Abstract: The present invention relates to analysis devices having means (3, 5, 7) for producing a plurality of ion beams of samples substantially simultaneously; mass separating means for individually mass separating each ion beam in parallel and detecting means (131-13n) for detecting said mass separated ion beams substantially simultaneously, and to methods for using such devices.

Abstract: A micro-channel plate (MCP) detector system (30) comprising a MCP detector, a data acquisition unit (20), wherein the detector comprises a first and a second MCP electron multiplier (12, 14), one or more anodes (16) connected to the data acquisition unit (20) and a gate electrode (32) disposed between the first and the second MCP electron multiplier (12, 14), wherein the detector system further comprises a data storage unit (36) and a gain control unit (34) which is connected to the gate electrode (32) and to the data storage unit (36), wherein a pilot spectrum is stored in the data storage unit (36), and wherein the gain control unit (34) is arranged to read the pilot spectrum from the data storage unit (36), and to control the potential on the gate electrode (32) as a function of m/z or time in response to said pilot spectrum, such that the transmission of electrons to the second MCP electron multiplier (14) is lowered when abundant protein ions appear, whereby a high sensitivity is maintained during the re

Abstract: This invention relates to the improvement of arrays of porous polymer pads on solid supports used in biological assays. The invention involves freeze drying the porous polymer pads to increase pore size. The increased pore size results in an enhanced ability of the porous polymer pads to bind specific binding substances such as DNA, RNA and polypeptides.