Patents Assigned to Amersham Life Science, Inc.
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Patent number: 5939257Abstract: Enzymatically active thermostable alkaline phosphatases from Rhodothermus marinus, Thermus thermophilus, and Thermosipho africanus.Type: GrantFiled: October 25, 1996Date of Patent: August 17, 1999Assignee: Amersham Life Science, Inc.Inventors: Joseph Szasz, Maria Davis
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Patent number: 5885813Abstract: An enzymatically active DNA polymerase having between 540 and 582 amino acids having a tyrosine at a position equivalent to position 667 of Taq DNA polymerase, wherein said polymerase lacks 5' to 3' exonuclease activity, and wherein said polymerase has at least 95% homology in its amino acid sequence to the DNA polymerase of Thermus aquaticus, Thermus flavus or Thermus thermophilus, and wherein said polymerase forms a single polypeptide band on an SDS PAGE.Type: GrantFiled: May 14, 1996Date of Patent: March 23, 1999Assignee: Amersham Life Science, Inc.Inventors: Maria Davis, R. Bruce Moffett, Carl W. Fuller, John J. Cunniff
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Patent number: 5849166Abstract: A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.Type: GrantFiled: April 16, 1996Date of Patent: December 15, 1998Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5830642Abstract: A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.Type: GrantFiled: August 10, 1992Date of Patent: November 3, 1998Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5827716Abstract: A Pol-II type DNA polymerase wherein an alanine located at the nucleotide binding site is replaced with a hydroxy containing amino acid.Type: GrantFiled: July 30, 1996Date of Patent: October 27, 1998Assignee: Amersham Life Science, Inc.Inventor: Joseph A. Mamone
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Patent number: 5756285Abstract: Method for determining the nucleotide base sequence of a DNA molecule by amplifying that DNA, contacting the products of the amplifying reaction with an alkaline phosphatase, and determining the nucleotide base sequence of any polynucleic acid products of the amplifying reaction.Type: GrantFiled: April 14, 1994Date of Patent: May 26, 1998Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5744312Abstract: An enzymatically active DNA polymerase or fragment thereof having at least 80% homology in its amino acid sequence to at least a contiguous 40 amino acid sequence of the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus.Type: GrantFiled: December 13, 1996Date of Patent: April 28, 1998Assignee: Amersham Life Science, Inc.Inventors: Joseph A. Mamone, Maria Davis, Dan Sha
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Patent number: 5741676Abstract: Amplified DNA is prepared for sequencing by contacting with exonuclease I and alkaline phosphatase which degrade undesirable excess primers dNTPs and non-specifically amplified single stranded DNA therein. The enzymes are provided in a kit.The method for sequencing DNA includes the following steps: providing a polynucleotide primer complementary to a region of the DNA, providing the DNA to be sequenced, and contacting that primer and DNA together in the presence of a DNA polymerase and between 1 and 3 dNTPs, at least one of the dNTP being labelled. The primer and DNA are contacted under conditions which allow extension of the primer by addition of one or more of the dNTPs to the primer to form an extended primer. The primer and DNA are then dissociated, generally by heating, and the contacting and dissociating steps repeated a plurality of times (usually 10-200 times).Type: GrantFiled: May 9, 1995Date of Patent: April 21, 1998Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5741640Abstract: Method for sequencing DNA which includes the following steps: providing a polynucleotide primer complementary to a region of the DNA to be sequenced providing the DNA to be sequenced, and contacting that primer and DNA together in the presence of a DNA polymerase and between 1 and 3 dNTPs, at least one of the dNTPs being labeled. The primer and DNA are contacted under conditions which allow extension of the primer by addition of one or more of the dNTPs to the primer to form an extended primer. The primer and DNA are then dissociated, generally by heating, and the contacting and dissociating steps repeated a plurality of times (usually 10-200 times). Finally, the extended primer is contacted with the DNA in the presence of a DNA polymerase (which is generally the same polymerase as used in the initial labeling step) all four dNTPs and a chain terminating agent.Type: GrantFiled: May 18, 1995Date of Patent: April 21, 1998Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5719056Abstract: Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-9.Type: GrantFiled: April 24, 1996Date of Patent: February 17, 1998Assignee: Amersham Life Science, Inc.Inventors: Shauna R. Brummet, Frank T. Robb, Kimberly M. Borges, Kristine M. Hujer, Sally T. Domke
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Patent number: 5674679Abstract: Method for sequencing DNA which includes the following steps: providing a polynucleotide primer complementary to a region of the DNA, providing the DNA to be sequenced, and contacting that primer and DNA together in the presence of a DNA polymerase and between 1 and 3 dNTPs, at least one of the dNTP being labelled. The primer and DNA are contacted under conditions which allow extension of the primer by addition of one or more of the dNTPs to the primer to form an extended primer. The primer and DNA are then dissociated, generally by heating, and the contacting and dissociating steps repeated a plurality of times (usually 10-200 times). Finally, the extended primer is contacted with the DNA in the presence of a DNA polymerase (which is generally the same polymerase as used in the initial labelling step) all four dNTPs and a chain terminating agent.Type: GrantFiled: September 27, 1991Date of Patent: October 7, 1997Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5633138Abstract: Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10.5 and is stable to heating at 65.degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.Type: GrantFiled: May 30, 1995Date of Patent: May 27, 1997Assignee: Amersham Life Science, Inc.Inventors: Maria Davis, Joseph Szasz
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Patent number: 5610066Abstract: Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-8.Type: GrantFiled: December 10, 1993Date of Patent: March 11, 1997Assignee: Amersham Life Science, Inc.Inventors: Carl W. Fuller, Joseph Szasz, Frank T. Robb, Kimberly M. Borges, Maria Davis
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Patent number: 5516633Abstract: Method for determining the nucleotide base sequence of DNA present in a lambda virus coat which is not replicable in a bacterial cell independent of any lambda DNA within that coat. The method includes preparing a lambda phage preparation containing the DNA, purifying the nucleic acid from the lambda phage to provide purified nucleic acid, and contacting that purified nucleic acid with a T7-type gene 6 exonuclease to allow the exonuclease to remove at least a portion of one strand of the DNA. The method further includes providing a primer able to hybridize with the other strand of the DNA complementary to the portion of the one strand and contacting the primer with the other strand of DNA in the presence of at least one deoxynucleoside triphosphate (dNTP) and at least one chain terminating agent (e.g., a dideoxynucleoside triphosphate, ddNTP) and a DNA polymerase, to allow the primer to be extended by the polymerase until extension is stopped by incorporation of the chain terminating agent.Type: GrantFiled: August 6, 1992Date of Patent: May 14, 1996Assignee: Amersham Life Science, Inc.Inventor: Carl W. Fuller
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Patent number: 5500339Abstract: Method for determining the nucleotide base sequence of a double-stranded closed circular DNA. The method includes heating a solution which includes the DNA in the presence of at least 20% glycerol and/or ethylene glycol to at least 80.degree. C. to provide denatured DNA, and then sequencing the denatured DNA.Type: GrantFiled: March 30, 1993Date of Patent: March 19, 1996Assignee: Amersham Life Science, Inc.Inventors: Carl W. Fuller, Denise A. Pisa-Williamson