Abstract: Improved loop-gap resonators applicable to Electron-Spin Resonance spectroscopy and to quantum computing employ interdigitated capacitor structures to dramatically increase the capacitance of the resonator, along with corresponding decreases in loop size to enable measurements of small-volume samples or individual quantum bits (qubits). The interdigitated-capacitor structures are designed to minimize parasitic inductance.

Abstract: Improved loop-gap resonators applicable to Electron-Spin Resonance spectroscopy and to quantum computing employ interdigitated capacitor structures to dramatically increase the capacitance of the resonator, along with corresponding decreases in loop size to enable measurements of small-volume samples or individual quantum bits (qubits). The interdigitated-capacitor structures are designed to minimize parasitic inductance.

Abstract: The invention provides in one embodiment a composition which is a linear DNA molecule having a desired replacement sequence, and second and third sequences substantially homologous to non-identical portions of the gene and having proximal and distal ends, the proximal ends flanking the desired replacement sequence and the distal ends having a terminating nucleotide analog at each end of the molecule. Another embodiment of the invention provides methods, by blocking the 3′ ends of transforming DNA with 2′3′ dideoxynucleotides, to reduce the frequency of end-mediated DNA insertion. These methods introduce only one copy of the selectable gene at the target locus to achieve a precise gene disruption, reducing or eliminating undesirable and multiple insertions that occur both non-homologously and at the targeted locus.

Abstract: The invention provides in one embodiment a composition which is a linear DNA molecule having a desired replacement sequence, and second and third sequences substantially homologous to non-identical portions of the gene and having proximal and distal ends, the proximal ends flanking the desired replacement sequence and the distal ends having a terminating nucleotide analog at each end of the molecule. Another embodiment of the invention provides methods, by blocking the 3′ ends of transforming DNA with 2′3′ dideoxynucleotides, to reduce the frequency of end-mediated DNA insertion. These methods introduce only one copy of the selectable gene at the target locus to achieve a precise gene disruption, reducing or eliminating undesirable and multiple insertions that occur both non-homologously and at the targeted locus.