Abstract: Maturation signals provided via cyclic adenosine monophosphate (cAMP)/Exchange proteins activated by cAMP (Epac) signaling during in vitro generation of blood cells from reprogrammed cells or pluripotent stem cells achieve superior function of hematopoietic cells differentiated from stem cells. The cAMP/Epac signaling enables an increased efficiency of production of precursor to blood and to blood cells. These generated blood cells can be utilized for therapeutics, treatments, disease prevention, drug discovery, personalized medicine, regenerative medicine, cell and tissue generation, universal donor banks and related methods and compositions.
Type:
Grant
Filed:
October 21, 2016
Date of Patent:
January 3, 2023
Assignee:
Amniotics AB
Inventors:
Shobhit Saxena, Roger Emanuel Rönn, Niels-Bjarne Woods
Abstract: The invention relates to method for the separation of cord blood endothelial cells from hematopoietic cells in a manner that preserves the hematopoietic cells' repopulating ability and primitivity, without significantly reducing the yield of hematopoietic cells. The said endothelial cells from cord blood have a utility in cell based therapeutics. The system described takes advantage of the endogenous adherence properties of the cells to facilitate the separation and separate storage of the cell types.
Type:
Grant
Filed:
March 14, 2014
Date of Patent:
August 4, 2020
Assignee:
Amniotics AB
Inventors:
Marcus Kare Torleif Larsson, Andreas Nils Walter Herbst, Niels-bjarne Roland Woods