Abstract: An embodiment relates to system comprising: (i) a reaction mixture comprises: a) a target nucleic acid molecule; b) a forward primer complementary to a strand of the target nucleic acid molecule, the forward primer comprises a first molecular moiety at a 3? end, wherein the first molecular moiety is non-complementary to the strand of the target nucleic acid molecule; c) a reverse primer complementary to a complementary sequence of the strand of the target nucleic acid molecule, the reverse primer comprises a second molecular moiety at a 3? end, wherein the second molecular moiety is non-complementary to the complementary sequence of the strand of the target nucleic acid molecule; d) a polymerase with 3?-5? exonuclease activity; and (ii a device suitable of detecting an amplification products.

Abstract: The disclosure provides compositions and methods for amplifying and/or analyzing nucleic acids.

Abstract: The disclosure provides compositions and methods for amplifying nucleic acids.