Patents Assigned to Amyris, Inc.
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Publication number: 20240099950Abstract: The disclosure provides cosmetic compositions including cannabigerol (CBG), as well as methods of using the same for reducing skin redness, improving skin barrier functionality, brightening the skin of a subject, and enhancing the outward appearance of the skin of a subject (e.g., a male or female human subject).Type: ApplicationFiled: January 11, 2022Publication date: March 28, 2024Applicant: Amyris, Inc.Inventors: Diva CHAN, Ro OTERI, Ashlee NUNES
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Patent number: 11884928Abstract: The invention provides methods for genetically engineering Kluyveromyces. The methods can be used to genetically engineer Kluyveromyces to produce and secrete full-length antibodies or antibody fragments. The invention also provides methods for production and secretion of antibodies.Type: GrantFiled: September 12, 2018Date of Patent: January 30, 2024Assignee: Amyris, Inc.Inventor: Yoseph Tsegaye
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Patent number: 11866738Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.Type: GrantFiled: July 22, 2021Date of Patent: January 9, 2024Assignee: AMYRIS, INC.Inventors: Lishan Zhao, Wenzong Li, Gale Wichmann, Aditi Khankhoje, Chantal Garcia De Gonzalo, Tina Mahatdejkul-Meadows, Shaina Jackson, Michael Leavell, Darren Platt
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Patent number: 11802100Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C50 terpenes), methods for making the same, and methods for their use.Type: GrantFiled: November 3, 2021Date of Patent: October 31, 2023Assignee: Amyris, Inc.Inventors: Nicholas Ohler, Karl Fisher, Jin Ki Hong
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Patent number: 11767533Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: GrantFiled: July 9, 2021Date of Patent: September 26, 2023Assignee: AMYRIS, INC.Inventors: Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao
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Patent number: 11725225Abstract: Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.Type: GrantFiled: September 19, 2008Date of Patent: August 15, 2023Assignee: AMYRIS, INC.Inventors: Hiroko Tsuruta, Jacob R. Lenihan, Rika Regentin
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Patent number: 11685934Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.Type: GrantFiled: September 12, 2018Date of Patent: June 27, 2023Assignee: Amyris, Inc.Inventor: Jessica Walter
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Patent number: 11390874Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.Type: GrantFiled: September 12, 2018Date of Patent: July 19, 2022Assignee: Amyris, Inc.Inventor: Hanxiao Jiang
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Patent number: 11390888Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. in certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. in some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: GrantFiled: March 11, 2020Date of Patent: July 19, 2022Assignee: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Patent number: 11312976Abstract: A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.Type: GrantFiled: June 27, 2019Date of Patent: April 26, 2022Assignee: AMYRIS, INC.Inventors: Ronald Ray Henry, Joshua Steven Leng
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Patent number: 11293033Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.Type: GrantFiled: May 18, 2017Date of Patent: April 5, 2022Assignee: AMYRIS, INC.Inventors: Kevin George, Andrew Main, Chia-Hong Tsai
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Patent number: 11230716Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.Type: GrantFiled: January 2, 2020Date of Patent: January 25, 2022Assignees: AMYRIS, INC., TOTAL MARKETING SERVICESInventors: Penelope R. Chua, Adam Meadows
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Patent number: 11219586Abstract: Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.Type: GrantFiled: July 14, 2021Date of Patent: January 11, 2022Assignee: Amyris, Inc.Inventors: Diva Chan, Ro Oteri, Ashlee Nunes
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Patent number: 11213467Abstract: Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.Type: GrantFiled: March 19, 2021Date of Patent: January 4, 2022Assignee: Amyris, Inc.Inventors: Diva Chan, Ro Oteri, Ashlee Nunes
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Patent number: 11193078Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C30 terpenes), methods for making the same, and methods for their use.Type: GrantFiled: April 19, 2019Date of Patent: December 7, 2021Assignee: AMYRIS, INC.Inventors: Nicholas Ohler, Karl Fisher, Jin Ki Hong
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Patent number: 11103808Abstract: The present invention is a process for recovering an isoprenoid compound produced by fermentation from a fermentation medium comprising: (a) demulsifying the fermentation medium or a fraction thereof in presence of a salt and a surfactant, thereby generating a stream having an organic phase containing the isoprenoid compound and a phase heavier than the organic phase, and (b) separating the stream obtained in step (a) into the organic phase containing the isoprenoid compound, the phase heavier than the organic phase, and optionally a solid phase containing host cell debris and cells.Type: GrantFiled: August 1, 2018Date of Patent: August 31, 2021Assignee: Amyris, Inc.Inventors: Maha Bahi, Anthony Hutin, Fernando Leal-Calderon
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Patent number: 11091743Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.Type: GrantFiled: August 11, 2017Date of Patent: August 17, 2021Assignee: AMYRIS, INC.Inventors: Lishan Zhao, Wenzong Li, Gale Wichmann, Aditi Khankhoje, Chantal Garcia De Gonzalo, Tina Mahatdejkul-Meadows, Shaina Jackson, Michael Leavell, Darren Platt
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Patent number: 11085046Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.Type: GrantFiled: October 28, 2016Date of Patent: August 10, 2021Assignee: AMYRIS, INC.Inventors: Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao
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Patent number: 10988513Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.Type: GrantFiled: June 24, 2016Date of Patent: April 27, 2021Assignees: AMYRIS, INC., TOTAL MARKETING SERVICESInventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
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Patent number: 10927382Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.Type: GrantFiled: August 7, 2013Date of Patent: February 23, 2021Assignees: AMYRIS, INC., TOTAL MARKETING SERVICESInventors: Hanxiao Jiang, Adam Meadows