Patents Assigned to Amyris, Inc.
  • Patent number: 12031169
    Abstract: Provided herein are compounds, compositions, and methods for recovery of one or more water-immiscible compounds from microbial biomass.
    Type: Grant
    Filed: April 25, 2022
    Date of Patent: July 9, 2024
    Assignee: Amyris, Inc.
    Inventors: Ronald Ray Henry, Joshua Steven Leng
  • Patent number: 11976190
    Abstract: The present invention relates to a block copolymer that contains a polymer block A containing a farnesene-derived monomer unit (a1) and a random copolymer block B containing a C12 or lower conjugated diene-derived monomer unit (b1) and an aromatic vinyl compound-derived monomer unit (b2), the block copolymer having a ratio by mass of the polymer block A to the random copolymer block B (A/B) of 30/70 to 0.5/99.5, a content of the aromatic vinyl compound-derived monomer unit (b2) in the random copolymer block B of 1 to 50% by mass, and a weight average molecular weight (Mw) of 100,000 to 5,000,000.
    Type: Grant
    Filed: March 28, 2019
    Date of Patent: May 7, 2024
    Assignees: KURARAY CO., LTD., AMYRIS, INC.
    Inventor: Daisuke Koda
  • Publication number: 20240099950
    Abstract: The disclosure provides cosmetic compositions including cannabigerol (CBG), as well as methods of using the same for reducing skin redness, improving skin barrier functionality, brightening the skin of a subject, and enhancing the outward appearance of the skin of a subject (e.g., a male or female human subject).
    Type: Application
    Filed: January 11, 2022
    Publication date: March 28, 2024
    Applicant: Amyris, Inc.
    Inventors: Diva CHAN, Ro OTERI, Ashlee NUNES
  • Patent number: 11884928
    Abstract: The invention provides methods for genetically engineering Kluyveromyces. The methods can be used to genetically engineer Kluyveromyces to produce and secrete full-length antibodies or antibody fragments. The invention also provides methods for production and secretion of antibodies.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: January 30, 2024
    Assignee: Amyris, Inc.
    Inventor: Yoseph Tsegaye
  • Patent number: 11866738
    Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
    Type: Grant
    Filed: July 22, 2021
    Date of Patent: January 9, 2024
    Assignee: AMYRIS, INC.
    Inventors: Lishan Zhao, Wenzong Li, Gale Wichmann, Aditi Khankhoje, Chantal Garcia De Gonzalo, Tina Mahatdejkul-Meadows, Shaina Jackson, Michael Leavell, Darren Platt
  • Patent number: 11802100
    Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C50 terpenes), methods for making the same, and methods for their use.
    Type: Grant
    Filed: November 3, 2021
    Date of Patent: October 31, 2023
    Assignee: Amyris, Inc.
    Inventors: Nicholas Ohler, Karl Fisher, Jin Ki Hong
  • Patent number: 11767533
    Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.
    Type: Grant
    Filed: July 9, 2021
    Date of Patent: September 26, 2023
    Assignee: AMYRIS, INC.
    Inventors: Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao
  • Patent number: 11725225
    Abstract: Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.
    Type: Grant
    Filed: September 19, 2008
    Date of Patent: August 15, 2023
    Assignee: AMYRIS, INC.
    Inventors: Hiroko Tsuruta, Jacob R. Lenihan, Rika Regentin
  • Patent number: 11685934
    Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: June 27, 2023
    Assignee: Amyris, Inc.
    Inventor: Jessica Walter
  • Patent number: 11390874
    Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: July 19, 2022
    Assignee: Amyris, Inc.
    Inventor: Hanxiao Jiang
  • Patent number: 11390888
    Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. in certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. in some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: July 19, 2022
    Assignee: AMYRIS, INC.
    Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
  • Patent number: 11312976
    Abstract: A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: April 26, 2022
    Assignee: AMYRIS, INC.
    Inventors: Ronald Ray Henry, Joshua Steven Leng
  • Patent number: 11293033
    Abstract: Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: April 5, 2022
    Assignee: AMYRIS, INC.
    Inventors: Kevin George, Andrew Main, Chia-Hong Tsai
  • Patent number: 11230716
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Grant
    Filed: January 2, 2020
    Date of Patent: January 25, 2022
    Assignees: AMYRIS, INC., TOTAL MARKETING SERVICES
    Inventors: Penelope R. Chua, Adam Meadows
  • Patent number: 11219586
    Abstract: Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
    Type: Grant
    Filed: July 14, 2021
    Date of Patent: January 11, 2022
    Assignee: Amyris, Inc.
    Inventors: Diva Chan, Ro Oteri, Ashlee Nunes
  • Patent number: 11213467
    Abstract: Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
    Type: Grant
    Filed: March 19, 2021
    Date of Patent: January 4, 2022
    Assignee: Amyris, Inc.
    Inventors: Diva Chan, Ro Oteri, Ashlee Nunes
  • Patent number: 11193078
    Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C30 terpenes), methods for making the same, and methods for their use.
    Type: Grant
    Filed: April 19, 2019
    Date of Patent: December 7, 2021
    Assignee: AMYRIS, INC.
    Inventors: Nicholas Ohler, Karl Fisher, Jin Ki Hong
  • Patent number: 11103808
    Abstract: The present invention is a process for recovering an isoprenoid compound produced by fermentation from a fermentation medium comprising: (a) demulsifying the fermentation medium or a fraction thereof in presence of a salt and a surfactant, thereby generating a stream having an organic phase containing the isoprenoid compound and a phase heavier than the organic phase, and (b) separating the stream obtained in step (a) into the organic phase containing the isoprenoid compound, the phase heavier than the organic phase, and optionally a solid phase containing host cell debris and cells.
    Type: Grant
    Filed: August 1, 2018
    Date of Patent: August 31, 2021
    Assignee: Amyris, Inc.
    Inventors: Maha Bahi, Anthony Hutin, Fernando Leal-Calderon
  • Patent number: 11091743
    Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: August 17, 2021
    Assignee: AMYRIS, INC.
    Inventors: Lishan Zhao, Wenzong Li, Gale Wichmann, Aditi Khankhoje, Chantal Garcia De Gonzalo, Tina Mahatdejkul-Meadows, Shaina Jackson, Michael Leavell, Darren Platt
  • Patent number: 11085046
    Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: August 10, 2021
    Assignee: AMYRIS, INC.
    Inventors: Andrew Main, Grzegorz Wojciechowski, Yue Yang, Lishan Zhao