Abstract: A capillary electrophoresis detection and/or analysis method comprising the following steps:rinsing a ready-to-use capillary with an initiator to create an initialized capillary,adding a capillary buffer into the initialized capillary,injecting a sample to be analyzed (possibly diluted with a sample diluent) into the initialized capillary,optionally, adding a cathodic buffer to the cathodic end of the capillary, andsubmitting the sample to capillary electrophoresis.In this method, the capillary and/or cathodic buffer comprise(s) a polyanion or a mixture of polyanions. The polyanion or the mixture of polyanions is included at least in the capillary or the cathodic buffer. The method can additionally comprise rinsing the capillary with NaOH after electrophoresis.The present invention also includes a capillary electrophoresis detection and/or analysis unit, including an initiator and a capillary buffer and/or a cathodic buffer.
Type:
Grant
Filed:
March 22, 1995
Date of Patent:
March 18, 1997
Assignee:
Analis S. A.
Inventors:
Jacques Janssens, Roland Chevigne, Philippe Louis
Abstract: The invention essentially relates to a method for the separation, identification and quantification of the isoenzymes and isoforms of alkaline phosphatase in samples of physiological fluid or tissue extracts, in which method a buffer, at least one nonionic detergent and at least one anionic detergent are applied to a support medium.Hepatic, osseous, placental, macromolecular and intestinal isoenzymes and isoenzymes bound to LpX, in particular, may be detected reliably while avoiding, as far as possible, the use of confirmation tests.
Abstract: According to the invention, an agarose gel containing chondroitin sulfate is used as a support medium for electrophoresis for separating glycosylated hemoglobin Hb Alc from the other types of hemoglobins.A densitometric analysis of the gel after electrophoresis enables the percentage of each type of hemoglobin contained in the sample to be quantified.