Abstract: Systems and methods for culturing and/or analyzing cells are provided. The system can include a microfluidic layer, a multi-well grid layer and a base layer. An electrode layer can optionally be provided. The system can also include an alignment feature for aligning microchannels of the microfluidic layer with electrodes of the electrode layer to achieve a predetermined organized architecture of the microchannels relative to the electrodes. The system can include a plurality of microfluidic layers and a microfluidic layer engaging frame engageable with the plurality of microfluidic layers to form a unitary structure engageable with a multi-well plate comprising a plurality of wells each comprising an electrode layer. A well identification feature associated with a microfluidic unit can be provided on an upper surface of the multi-grid layer to enable visual identification of a well of the multi-well grid layer that is in fluid communication with a microfluidic unit.