Patents Assigned to Anaphore, Inc.
  • Patent number: 8318679
    Abstract: The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.
    Type: Grant
    Filed: March 16, 2009
    Date of Patent: November 27, 2012
    Assignees: Anaphore, Inc., Hoffman-La Roche, Inc.
    Inventors: Hans Christian Thøgersen, Michael Etzerodt, Thor Las Holtet, Niels Jonas Heilskov Graversen, Jette Sandholm Kastrup, Bettina Bryde Nielsen, Ingrid Kjøller Larsen
  • Patent number: 8192953
    Abstract: The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.
    Type: Grant
    Filed: March 16, 2009
    Date of Patent: June 5, 2012
    Assignees: Anaphore, Inc., Hoffmann-La Roche, Inc.
    Inventors: Hans Christian Thøgersen, Michael Etzerodt, Thor Las Holtet, Niels Jonas Heilskov Graversen, Jette Sandholm Kastrup, Bettina Bryde Nielsen, Ingrid Kjøller Larsen
  • Patent number: 8017559
    Abstract: The present invention provides novel polypeptides having the scaffold structure of a C-type lectin-like domain (CTLD) and a randomized loop region for specifically binding a variety of target compounds and also provides nucleic acids encoding the polypeptides. The present invention further provides combinatorial CTLD libraries, methods for constructing the libraries, and methods for screening the libraries to identify and isolate the novel CTLD polypeptides. Specifically, the invention provides libraries of nucleic acids encoding polypeptides having a scaffold CTLD with a randomized loop region, as well as nucleic acid sequences, vectors, and methods for preparing and expressing the libraries. Exemplary nucleic acids useful in the combinatorial libraries are derived from tetranectin and other proteins having a CTLD.
    Type: Grant
    Filed: December 4, 2006
    Date of Patent: September 13, 2011
    Assignee: Anaphore, Inc.
    Inventors: Michael Etzerodt, Thor Las Holtet, Niels Jonas Heilskov Graversen, Hans Christian Thøgersen
  • Patent number: 7820622
    Abstract: TNF binding polypeptides based on human tetranectin C-type lectin like domains (CTLD) with improved binding characteristics and improved efficacy. The polypeptides comprise a TNF binding domain having the amino acid sequence KRWS-RYF (SEQ ID NO:1). Also provided are methods of preparing the polypeptides of the invention. The polypeptides may be used for the preparation of pharmaceutical compositions, and for treatment of a subject having a pathology mediated by TNF, such as treatment of rheumatoid arthritis.
    Type: Grant
    Filed: November 21, 2005
    Date of Patent: October 26, 2010
    Assignee: Anaphore, Inc.
    Inventors: Helle Krogh Ottow, Mette Munch, Thor Las Holtet, Mikkel Holmen Andersen, Josephus Dirk Nieland
  • Patent number: 7642044
    Abstract: The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.
    Type: Grant
    Filed: June 14, 2006
    Date of Patent: January 5, 2010
    Assignees: Anaphore, Inc., Hoffman-La Roche, Inc.
    Inventors: Hans Christian Thogersen, Michael Etzerodt, Thor Las Holtet, Niels Jonas Heilskov Graversen, Jette Sandholm Kastrup, Bettina Bryde Nielsen, Ingrid Kjoller Larsen
  • Publication number: 20090155843
    Abstract: TNF binding polypeptides based on human tetranectin C-type lectin like domains (CTLD) with improved binding characteristics and improved efficacy. The polypeptides comprise a TNF binding domain having the amino acid sequence KRWS-RYF (SEQ ID NO:1). Also provided are methods of preparing the polypeptides of the invention. The polypeptides may be used for the preparation of pharmaceutical compositions, and for treatment of a subject having a pathology mediated by TNF, such as treatment of rheumatoid arthritis.
    Type: Application
    Filed: November 21, 2005
    Publication date: June 18, 2009
    Applicant: Anaphore, Inc.
    Inventors: Helle Krogh Ottow, Mette Munch, Thor Las Holtet, Mikkel Holmen Andersen, Josephus Dirk Nieland