Patents Assigned to ANDE Corporation
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Patent number: 11900604Abstract: Described herein is an adaptive expert system comprising a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions. The adaptive expert system receives sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA. The adaptive expert system determines at least one characteristic of the sample data. The adaptive expert system utilizes the at least one characteristic to classify the sample data and apply a predefined parameter set to the said sample data to generate an output.Type: GrantFiled: July 12, 2018Date of Patent: February 13, 2024Assignee: ANDE CorporationInventors: Eugene Tan, Hua Jiang, Richard F. Selden
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Publication number: 20230394665Abstract: Described herein is an adaptive expert system comprising a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions. The adaptive expert system receives sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA. The adaptive expert system determines at least one characteristic of the sample data. The adaptive expert system utilizes the at least one characteristic to classify the sample data and apply a predefined parameter set to the said sample data to generate an output.Type: ApplicationFiled: August 17, 2023Publication date: December 7, 2023Applicant: Ande CorporationInventors: Eugene Tan, Hua Jiang, Richard F. Selden
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Patent number: 11612893Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: GrantFiled: December 2, 2016Date of Patent: March 28, 2023Assignee: ANDE CORPORATIONInventors: Richard F. Selden, Eugene Tan
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Patent number: 11612894Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: GrantFiled: January 26, 2017Date of Patent: March 28, 2023Assignee: ANDE CORPORATIONInventors: Richard F. Selden, Eugene Tan
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Publication number: 20220389487Abstract: Described herein are instruments for excitation and detection of fluorophores in a plurality of functional regions in a biochip, using an excitation source and a steering element that directs a beam from the excitation source to a plurality of functional regions in the biochip, wherein the excitation source excites the fluorophores in the plurality of functional regions generating a signal that is detected such that said signal from at least one of the plurality of functional regions allows for nucleic acid quantification. Also described are systems for quantification and separation and detection using optical devices adapted for preliminary, simultaneous or sequential quantitation of nucleic acid in separate detection positions, and for the excitation and detection of multiple samples to steer both the excitation and detection beam paths to separately image each lane of a biochip.Type: ApplicationFiled: August 8, 2022Publication date: December 8, 2022Applicant: ANDE CorporationInventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
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Publication number: 20220315996Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.Type: ApplicationFiled: June 9, 2022Publication date: October 6, 2022Applicant: ANDE CORPORATIONInventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
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Patent number: 11441173Abstract: Described herein are instruments for excitation and detection of fluorophores in a plurality of functional regions in a biochip, using an excitation source and a steering element that directs a beam from the excitation source to a plurality of functional regions in the biochip, wherein the excitation source excites the fluorophores in the plurality of functional regions generating a signal that is detected such that said signal from at least one of the plurality of functional regions allows for nucleic acid quantification. Also described are systems for quantification and separation and detection using optical devices adapted for preliminary, simultaneous or sequential quantitation of nucleic acid in separate detection positions, and for the excitation and detection of multiple samples to steer both the excitation and detection beam paths to separately image each lane of a biochip.Type: GrantFiled: December 27, 2019Date of Patent: September 13, 2022Assignee: ANDE CORPORATIONInventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
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Systems and methods for rapid nucleic acid extraction, purification and analysis from bone and tooth
Patent number: 11168318Abstract: Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.Type: GrantFiled: February 15, 2018Date of Patent: November 9, 2021Assignee: ANDE CORPORATIONInventors: Richard F. Selden, Rosemary Turingan Witkowski -
Patent number: 11110461Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.Type: GrantFiled: February 12, 2018Date of Patent: September 7, 2021Assignee: ANDE CORPORATIONInventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
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Patent number: 11052398Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.Type: GrantFiled: June 7, 2018Date of Patent: July 6, 2021Assignee: ANDE CORPORATIONInventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
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Patent number: 11022555Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.Type: GrantFiled: October 16, 2017Date of Patent: June 1, 2021Assignee: ANDE CORPORATIONInventors: James W. Schumm, Richard F. Selden, Eugene Tan
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Patent number: 10821445Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: GrantFiled: May 2, 2016Date of Patent: November 3, 2020Assignee: ANDE CorporationInventors: Richard F. Selden, Eugene Tan
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Patent number: 10538804Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.Type: GrantFiled: January 19, 2017Date of Patent: January 21, 2020Assignee: ANDE CorporationInventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
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Patent number: 10464065Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.Type: GrantFiled: October 3, 2016Date of Patent: November 5, 2019Assignee: ANDE CorporationInventors: Richard F. Selden, Eugene Tan
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Patent number: 9994895Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.Type: GrantFiled: January 13, 2015Date of Patent: June 12, 2018Assignee: ANDE CORPORATIONInventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
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Patent number: 9889449Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.Type: GrantFiled: May 10, 2016Date of Patent: February 13, 2018Assignee: ANDE CorporationInventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
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Patent number: 9797841Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.Type: GrantFiled: March 14, 2013Date of Patent: October 24, 2017Assignee: ANDE CorporationInventors: James W. Schumm, Richard F. Selden, Eugene Tan