Patents Assigned to Anderson Forschung Group
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Patent number: 10345293Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.Type: GrantFiled: July 13, 2017Date of Patent: July 9, 2019Assignee: Anderson Forschung Group LLCInventor: Norman Leigh Anderson
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Patent number: 9970943Abstract: Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.Type: GrantFiled: February 29, 2016Date of Patent: May 15, 2018Assignee: Anderson Forschung Group, LLCInventor: N. Leigh Anderson
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Patent number: 9746464Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.Type: GrantFiled: October 19, 2015Date of Patent: August 29, 2017Assignee: ANDERSON FORSCHUNG GROUP, LLC.Inventor: Norman Leigh Anderson
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Publication number: 20170138955Abstract: A method for determining the concentration ratio in a sample of a target peptide to a reference peptide that is chemically identical with the target peptide, but labeled by isotopes, acquires mass spectra of the target and reference peptides. One of a plurality of families of superimposed bell-shaped curves which is a best fit to ion current peak groups of the target and reference peptides in the mass spectra is determined by varying parameters of the families. In each family, each bell-shaped curve has a predetermined height, the curves have fixed distances from each other and the relative curve heights and curve distances in the families are individually calculated from an elemental composition of the peptides and an isotope abundance distribution of elements composing the peptides, taking into account purity of the isotopes. The concentration ratio is then determined from the parameters of the best fit.Type: ApplicationFiled: January 27, 2017Publication date: May 18, 2017Applicants: Bruker Daltonik GmbH, Anderson Forschung Group LLCInventors: Leigh ANDERSON, Detlev SUCKAU
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Patent number: 9581589Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.Type: GrantFiled: December 1, 2009Date of Patent: February 28, 2017Assignee: ANDERSON FORSCHUNG GROUP LLCInventor: Norman L. Anderson
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Patent number: 9558922Abstract: A method for determining the concentration ratio in a sample of a target peptide to a reference peptide that is chemically identical with the target peptide, but labeled by isotopes, acquires mass spectra of the target and reference peptides. One of a plurality of families of superimposed bell-shaped curves which is a best fit to ion current peak groups of the target and reference peptides in the mass spectra is determined by varying parameters of the families. In each family, each bell-shaped curve has a predetermined height, the curves have fixed distances from each other and the relative curve heights and curve distances in the families are individually calculated from an elemental composition of the peptides and an isotope abundance distribution of elements composing the peptides, taking into account purity of the isotopes. The concentration ratio is then determined from the parameters of the best fit.Type: GrantFiled: May 31, 2012Date of Patent: January 31, 2017Assignees: Bruker Daltonik GmbH, Anderson Forschung Group LLCInventors: Leigh Anderson, Detlev Suckau
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Publication number: 20160282361Abstract: Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.Type: ApplicationFiled: February 29, 2016Publication date: September 29, 2016Applicant: ANDERSON FORSCHUNG GROUP, INC.Inventor: N. Leigh ANDERSON
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Patent number: 9274124Abstract: Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.Type: GrantFiled: March 15, 2011Date of Patent: March 1, 2016Assignee: ANDERSON FORSCHUNG GROUP, INC.Inventor: Norman Leigh Anderson
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Patent number: 9164089Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.Type: GrantFiled: July 17, 2013Date of Patent: October 20, 2015Assignee: Anderson Forschung Group LLCInventor: N. Leigh Anderson
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Publication number: 20140120549Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.Type: ApplicationFiled: July 17, 2013Publication date: May 1, 2014Applicant: ANDERSON FORSCHUNG GROUP, INC.Inventor: N. Leigh Anderson
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Publication number: 20130040857Abstract: Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.Type: ApplicationFiled: March 15, 2011Publication date: February 14, 2013Applicant: ANDERSON FORSCHUNG GROUP, INC.Inventor: Norman Leigh Anderson
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Publication number: 20100311097Abstract: This invention relates to proteins having an amino acid sequence containing several amino acid subsequences found in nature and wherein at least two different subsequences act as monitor sequences, said subsequences being part of at least one natural protein which is a target protein, wherein the end of each of said two different subsequences have a cleavage site that will be cleaved by the same site-specific proteolytic treatment to release said subsequences.Type: ApplicationFiled: February 2, 2010Publication date: December 9, 2010Applicant: ANDERSON FORSCHUNG GROUP LLCInventor: Norman L. Anderson
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Patent number: 7632686Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.Type: GrantFiled: October 2, 2003Date of Patent: December 15, 2009Assignee: Anderson Forschung GroupInventor: Norman L. Anderson