Abstract: A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culturing after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).
Type:
Grant
Filed:
June 13, 2013
Date of Patent:
January 2, 2018
Assignee:
ANGANY GENETICS
Inventors:
Veronique Gomord, Anne Catherine Fitchette, Loic Faye
Abstract: A plant cell including a DNA molecule having at least one heterologous nucleotide sequence encoding a preproprotein of a pectate lyase chosen from Amb a 1, the alpha subunit of Amb a 1, the beta subunit of Amb a 1, and homologs of Amb a 1, functionally bound to a strong promoter.
Abstract: A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably Nicotiana benthamiana, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culturing after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).
Type:
Application
Filed:
June 13, 2013
Publication date:
December 17, 2015
Applicant:
ANGANY GENETICS
Inventors:
Veronique GOMORD, Anne Catherine FITCHETTE, Loic FAYE