Abstract: The present application discloses a semi-dominant molecular marker related to maize dwarf and an application thereof, and relates to the technical field of maize molecular breeding. The molecular marker related to the maize dwarf is caused by a non-synonymous mutation from G to A in a maize gene Zm00001d013465, and the molecular marker is a nucleotide sequence composed of the non-synonymous mutation site and its upstream and downstream bases. The present application identifies a dwarf mutant, coded as E5779, from an EMS mutation population with maize B73 as the genetic background. By genome resequencing and Mutmap mapping, it is found that the mutant is caused by the non-synonymous mutation from G to A in the gene Zm00001d013465. The molecular marker is developed based on this, and the analysis and utilization of E5779 are helpful to improve the maize plant height and density-tolerant breeding, it has an important breeding application potential.

Abstract: A long-distance sampling device for feces in a poultry house is provided. The device includes an unmanned aerial vehicle body including a wireless controller, supporting feet arranged on the unmanned aerial vehicle body, and a sampling unit arranged on the unmanned aerial vehicle body for collecting fecal samples. The sampling unit further includes a sampling power portion arranged in the unmanned aerial vehicle body and a sampling portion arranged on the unmanned aerial vehicle body. The sampling portion further includes a cylindrical sampling tube and a sampling shaft rotatably connected to the sampling tube, spiral blades are fixedly connected to the sampling shaft, and the sampling power portion drives the sampling shaft to rotate. According to the device, it is convenient for workers to take samples in the poultry house, thereby the convenience of sampling is improved, and the workers do not need to get into the breeding areas.

Abstract: The present disclosure discloses a method for prolonging half-life of theanine in vivo. According to the present disclosure, a specific theanine glycoside compound formed by modification with a specific monosaccharide is used for prolonging the half-life of the theanine in vivo, which can be used for preparing sustained release drugs, prolonging action time of drugs, reducing administration frequency and improving compliance of patients. With the method of the present disclosure, the theanine glycoside compound obtained after modification can have a broad application prospect and a high commercial value in scientific research and clinical application.

Abstract: A graft copolymer and a preparation method and use thereof. The method for preparing a graft copolymer includes: dissolving an N-ethyl-2-pyrrolidinone-substituted flavan-3-ol (EPSF) and a starch in a polar organic solvent, to obtain a raw material solution; and mixing the raw material solution with ascorbic acid and hydrogen peroxide to obtain a mixture, and subjecting the mixture to a grafting reaction, to obtain the graft copolymer.

Abstract: The present disclosure relates to the technical fields of agriculture and environmental microbiology, and provides Agrobacterium for reducing uptake of heavy metals by wheat. The Agrobacterium is strain NJ20, which is deposited in China General Microbiological Culture Collection Center under Accession CGMCC No. 26197. The present disclosure also provides a bacterial agent which is prepared by the aforementioned Agrobacterium. The Agrobacterium and bacterial agent thereof are used for reducing uptake of heavy metals in heavy metal contaminated soil by wheat. The strain NJ20 of the present disclosure can grow well under the stress of lead and cadmium, and remove soluble lead and cadmium in the culture solution by adsorption; meanwhile the strain NJ20 can generate indoleacetic acid, dissolve insoluble tricalcium phosphate and improve phosphorus nutrition in soil. Inoculation of the strain NJ20 into heavy metal contaminated soil can reduce the contents of lead and cadmium in wheat grains and straws.

Abstract: The present disclosure discloses a novel strain of Glutamicibacter, derived from insects, which efficiently degrades bifenthrin, belonging to the field of microbial strains. The Glutamicibacter CCTCC NO: M20221445 of the present disclosure was isolated from the intestinal tract of bifenthrin-resistant Ectropis grisescens Warren larvae. It exhibits unique genomic characteristics, growth and phenotypic traits, physiological and biochemical characteristics, as well as the ability to utilize and degrade bifenthrin efficiently. Specifically, it can effectively degrade bifenthrin. Based on phenotypic features, physiological and biochemical characteristics, chemical composition, and molecular biology-based polyphasic classification, Glutamicibacter CCTCC NO: M20221445 is identified as a new species. This bacterium possesses the capability to efficiently degrade bifenthrin, laying the foundation for biological control of E. grisescens and offering new microbial resources to address pesticide residue problems.

Abstract: The present disclosure provides an antimicrobial peptide (AMP) for controlling Pseudomonas syringae pv. actinidiae (PSA) and a preparation method and use thereof, belonging to the technical field of biological control. In the present disclosure, the AMP has an amino acid sequence of Trp-Trp-Lys-Leu-Leu-Arg-Lys-Leu-NH2, and has a typical amphiphilic structure. The AMP can target a cell membrane of the PSA, increasing permeabilization and dissipating a membrane potential, which leads to calcium ion leakage. The AMP also acts on intracellular targets, affects DNA functions, reduces an activity of esterases, and induces the generation of reactive oxygen species (ROS). The AMP has a minimal inhibitory concentration (MIC) of 3.13 ?g/mL and a half maximal effective concentration (EC50) of 1.67 ?g/mL for the PSA, showing an activity significantly higher than that of agricultural streptomycin.

Abstract: A method for determining a grade of black tea by HPLC detection belongs to the field of tea grade determination. The specific steps are as follows: adding known black tea powder samples of different grades into boiling water of 95-100° ° C. for extraction, and filtering with a filter membrane with a pore size in a range of 0.20-0.25 um to obtain black tea sample liquid; measuring contents of ten components by peak area normalization method; standardizing data of the contents of the ten components in a black tea sample solution; carrying out unsupervised principal component analysis; carrying out supervised partial least squares discriminant analysis; carrying out hierarchical clustering analysis on the basis of partial least squares discriminant analysis, and finally establishing a tea grade discrimination model based on HPLC.

Abstract: The present disclosure belongs to the field of livestock molecular biotechnology, and provides kits and methods for pedigree division and paternity testing of domestic pigs. The kits and methods specifically select 14 SSR loci of domestic pigs, especially Anqing six-end-white pigs, and synthesize primers for corresponding loci. Through capillary electrophoresis detection of the ear tissue DNA of 98 Anqing six-end-white pigs, the count of effective alleles, heterozygosity, polymorphism information content, and genetic distance, and exclusion probability at each locus are calculated, the pedigree division of domestic pigs, especially Anqing six-end-white pigs, and paternity testing are conducted. The cumulative exclusion probability of 14 microsatellite loci is 99%.

Abstract: A sowing device changes a depth of wheat sowing includes an external skeleton, where one side of the external skeleton is fixedly connected with a connection part for articulated with a tractor; a main board slidably connected to an interior of the external skeleton and a seeding unit below the main board; a support frame fixedly connected to the main board; a plow blade for slotting land rotatably connected to a bottom of the support frame; a rotary roller below the external skeleton and rotationally connected thereto. The main board is moved downwards to drive a straight toothed plate fixedly connected to the main board to move downwards; the straight toothed plate is moved upwards through a meshing effect of a gear to cause L-shaped toothed plate to move upwards to squeeze a horizontal plate to move upwards.

Abstract: A graft copolymer and a preparation method and use thereof. The method for preparing a graft copolymer includes: dissolving an N-ethyl-2-pyrrolidinone-substituted flavan-3-ol (EPSF) and a starch in a polar organic solvent, to obtain a raw material solution; and mixing the raw material solution with ascorbic acid and hydrogen peroxide to obtain a mixture, and subjecting the mixture to a grafting reaction, to obtain the graft copolymer.

Abstract: A welfare-oriented raising house for meat-type ducks, including a raising house, is provided. The raising house is internally provided with a support frame which is of a U-shaped structure and arranged in a length direction of the raising house, a raising net arranged at a top of the support frame, a cleaning and storing mechanism arranged at one end of the support frame for cleaning and storing the raising net, a traction mechanism arranged at the other end of the support frame for the traction of the raising net, blocking mechanisms which are of a zigzag structure and arranged at both sides of the support frame, and tensioning vibration mechanisms arranged at bottoms of the blocking mechanisms and fixedly connected to a side edge of the support frame.

Abstract: A method for efficiently purifying and detecting 6-hydroxynobilonine in fresh stems of Dendrobium huoshanense uses a gas chromatography-mass spectrometry (GC-MS) method to detect 6-hydroxynobilonine in Dendrobium huoshanense is, i.e.

Abstract: Disclosed is a top surface wave antenna of a spherical Tokamak, comprising a feedback waveguide, a brim, sub-waveguides, and a metal base. The lower end of the feed waveguide is connected to one end of the metal base, and one side of the feed waveguide is connected to the brim. The brim is towards a length direction of the metal base. A plurality of sub-waveguides are arranged on the metal base at equal intervals, the tops of the sub-waveguides are not higher than the height of the metal base, and the sub-waveguides are arranged in a rising line trend. The feed waveguide serves as a microwave input port. The top surface wave antenna of the spherical Tokamak is mainly used in a high-power Tokamak system and acts on the low-hybrid wave current driving together with an external antenna so as to obtain a better effect.

Abstract: Disclosed is a Tokamak outer antenna, comprising feed waveguides, brims, sub-waveguides and a metal base. The metal base is in a W shape, both ends of the metal base are connected to the lower surfaces of feed waveguides, respectively. Opposite sides of two feed waveguides are connected to the brims, respectively, and a plurality of sub-waveguides are arranged on the upper surface of the metal base at equal intervals. The height of each sub-waveguide is not higher than the height of the feed waveguide, one feed waveguide serves as a microwave input port, and the other feed waveguide serves as a microwave output port. The Tokamak outer antenna disclosed by the present disclosure is a novel antenna which is simple in feed, low in reflection and transmission coefficients and high in directivity.

Abstract: The present disclosure provides a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide gene NPY, a method and use thereof. The NPY gene has a nucleotide sequence shown in SEQ ID NO: 1, the molecular marker is T or C, and the molecular marker is located at position 577 of the nucleotide sequence. In the present disclosure, mutation of the NPY gene is detected using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, the traits of efficiency of duck feed utilization are selected based on genotypes, and a breeding method for early selection of efficiency of poultry feed utilization is established. The method is simple, rapid, and low-cost, does not need special instrument, and satisfies experimental needs.

Abstract: The present disclosure provides a pyraclostrobin microcapsule based on sodium alginate, which is prepared by mixing pyraclostrobin original drug dissolved in ethyl acetate, 1% aqueous sodium alginate solution, and 5% aqueous calcium carbonate solution at 25° C., and mixing the mixed solution with liquid paraffin containing 10% emulsifier A-110, maintaining a shear rate of 1000 r/min to emulsify for 10 min; using 87.5 mmol/L glacial acetic acid to reduce the pH of the mixed phase, such that the cross-linking reaction between ions can be formed into microcapsules; finally using pH 4.5 acetate buffer to wash and separate the microcapsule slurry, and then centrifuging and freeze-drying to prepare the pyraclostrobin SA microcapsules. The microcapsules prepared by the present disclosure have excellent formulation performance and can be used for the control of Fusarium pseudograminearum.

Abstract: A method for efficiently purifying and detecting 6-hydroxynobilonine in fresh stems of dendrobium huoshanense uses a gas chromatography-mass spectrometry (GC-MS) method to detect 6-hydroxynobilonine in dendrobium huoshanense is, i.e.

Abstract: The present disclosure provides a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide gene NPY, a method and use thereof. The NPY gene has a nucleotide sequence shown in SEQ ID NO: 1, the molecular marker is T or C, and the molecular marker is located at position 577 of the nucleotide sequence. In the present disclosure, mutation of the NPY gene is detected using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, the traits of efficiency of duck feed utilization are selected based on genotypes, and a breeding method for early selection of efficiency of poultry feed utilization is established. The method is simple, rapid, and low-cost, does not need special instrument, and satisfies experimental needs.

Abstract: A growth-promoting bacterial agent capable of improving content of soybean oil, preparation method, and use thereof are provided. The growth-promoting bacterial agent is fermented by a DW1 strain, the classification name of the DW1 strain is Bacillus sp., the DW1 strain is deposited in the China Center for Type Culture Collection on Jul. 16, 2021, and the deposit number is CCTCC NO: M 2021889. The growth-promoting bacterial agent of the present disclosure has the ability to dissolve insoluble phosphorus and insoluble potassium and can increase content of readily available phosphorus and readily available potassium in the soil, which has an obvious promoting effect on the growth of soybean and improves soybean yield. The growth-promoting bacterial agent of the present disclosure promotes bacteria and can significantly improve the content of soybean oil. At the same time, it has an affinity of soybean agglutinin and can agglutinate with the soybean agglutinin.