Patents Assigned to Anima Cell Metrology
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Patent number: 10203335Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.Type: GrantFiled: February 9, 2017Date of Patent: February 12, 2019Assignees: The Trustees of the University of Pennsylvania, Anima Cell MetrologyInventors: Barry S. Cooperman, Zeev Smilansky, Yale E. Goldman, Dongli Pan
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Patent number: 9612244Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.Type: GrantFiled: June 3, 2014Date of Patent: April 4, 2017Assignees: The Trustees Of The University Of Pennsylvania, Anima Cell Metrology, Inc.Inventors: Barry S. Cooperman, Zeev Smilansky, Yale E. Goldman, Dongli Pan
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Patent number: 9377453Abstract: There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided methods and systems for the generation of a stress index of a living cell. There are further provided methods and systems for detection of stress in a living cell, comprising detection of changes in subcellular localization of labeled tRNA in a cell, wherein the detection is performed in real time.Type: GrantFiled: August 19, 2015Date of Patent: June 28, 2016Assignees: RAMOT at Tel Aviv University Ltd., Anima Cell Metrology, Inc.Inventors: Orna Elroy-Stein, Marcelo Ehrlich, Sima Barhoom, Zeev Smilansky
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Patent number: 9140650Abstract: There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided methods and systems for the generation of a stress index of a living cell. There are further provided methods and systems for detection of stress in a living cell, comprising detection of changes in subcellular localization of labeled tRNA in a cell, wherein the detection is performed in real time.Type: GrantFiled: July 21, 2011Date of Patent: September 22, 2015Assignees: Ramot at Tel Aviv University Ltd., Anima Cell Metrology, Inc.Inventors: Orna Elroy-Stein, Marcelo Ehrlich, Sima Barhoom, Zeev Smilansky
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Patent number: 9034576Abstract: The present invention relates to systems and methods for measuring the rate of translation of a target protein in cells, which are based on the detection of translation of one or more predetermined codon pairs during synthesis of the target protein. The detection is provided by a FRET signal emitted from labeled tRNA molecules which are juxtaposed during synthesis of the protein.Type: GrantFiled: September 21, 2010Date of Patent: May 19, 2015Assignee: ANIMA CELL METROLOGY INC.Inventors: Zeev Smilansky, Barry S. Cooperman, Yale E. Goodman
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Patent number: 9012171Abstract: Systems for measuring protein translation and methods for measuring overall translation activity in viable cells or subcellular compartments is disclosed. The methods identify general ribosomal activity, if desired at sub-cellular resolution, thereby providing a signal indicating the rate of any of the steps of protein synthesis selected from initiation, elongation, termination or recycling. The translation system can be used to identify translation modulators in high-throughput-screening (HTS).Type: GrantFiled: October 7, 2008Date of Patent: April 21, 2015Assignee: Anima Cell Metrology, Inc.Inventor: Ze'ev Smilansky
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Patent number: 9012150Abstract: Methods for identifying ribonucleotide sequences, in vitro, using the ribosome-mediated translation, are provided.Type: GrantFiled: May 26, 2005Date of Patent: April 21, 2015Assignee: Anima Cell MetrologyInventor: Ze'ev Smilansky
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Patent number: 8785119Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.Type: GrantFiled: June 20, 2008Date of Patent: July 22, 2014Assignees: Anima Cell Metrology, The Trustees of the University of PennsylvaniaInventors: Barry S. Cooperman, Zeev Smilansky, Yale E. Goldman, Dongli Pan
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Publication number: 20130122491Abstract: There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided methods and systems for the generation of a stress index of a living cell. There are further provided methods and systems for detection of stress in a living cell, comprising detection of changes in subcellular localization of labeled tRNA in a cell, wherein the detection is performed in real time.Type: ApplicationFiled: July 21, 2011Publication date: May 16, 2013Applicants: ANIMA CELL METROLOGY, INC., RAMOT AT TEL AVIV UNIVERSITY LTD.Inventors: Orna Elroy-Stein, Marcelo Ehrlich, Sima Barhoom, Zeev Smilansky
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Patent number: 8442773Abstract: A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome, wherein the ribosome is bound to a first label, for example a donor fluorophore, and a tRNA and/or amino acid are is bound to a second label, for example an acceptor fluorophore, wherein the first and second labels together from a FRET pair. As the ribosome mechanism processes the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for database searching and identification of the protein being synthesized.Type: GrantFiled: August 31, 2010Date of Patent: May 14, 2013Assignee: Anima Cell MetrologyInventor: Zeev Smilansky
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Publication number: 20120183957Abstract: The present invention relates to systems and methods for measuring the rate of translation of a target protein in cells, which are based on the detection of translation of one or more predetermined codon pairs during synthesis of the target protein. The detection is provided by a FRET signal emitted from labeled tRNA molecules which are juxtaposed during synthesis of the protein.Type: ApplicationFiled: September 21, 2010Publication date: July 19, 2012Applicant: ANIMA CELL METROLOGY, INC.Inventors: Zeev Smilansky, Barry S. Cooperman, Yale E. Goodman
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Publication number: 20110262899Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.Type: ApplicationFiled: June 20, 2008Publication date: October 27, 2011Applicants: Anima Cell Metrology, The Trustees of the University of PennsylvaniaInventors: Barry S. Cooperman, Zeev Smilansky, Yale E. Goldman, Dongli Pan
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Patent number: 7807349Abstract: A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome in real time, in vivo as well as in in-vitro. The ribosome is engineered to carry a donor fluorophore, and tRNA and/or amino acids and/or some other part of the ribosome are either engineered to carry acceptor fluorophores or else their natural fluorescent properties are utilized as acceptors. As the ribosomes mechanism processed the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for real-time database searching and identification of the protein being synthesized. The resulting data can be tabulated and interpreted in different ways. FIG. (1) describes the properties of a FRET pair and the dependence of FRET on pair distance.Type: GrantFiled: November 27, 2003Date of Patent: October 5, 2010Assignee: Anima Cell MetrologyInventor: Zeev Smilansky
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Publication number: 20090081643Abstract: Methods for identifying ribonucleotide sequences, in vitro, using the ribosome-mediated translation, are provided.Type: ApplicationFiled: May 26, 2005Publication date: March 26, 2009Applicant: ANIMA CELL METROLOGYInventors: Michal Preminger, Ze'ev Smilansky