Abstract: Provided herein are a hybridoma cell line producing monoclonal antibody against foot-and-mouth disease virus (FMDV), the monoclonal antibody therefrom, reagent and kit for ELISA, and immunoassay method. The hybridoma cell line CmA40 as deposited under American Type Culture Collection patent deposit number PTA-11304 is produced by cell fusion of a parental cell and a myeloma cell line. The parental cell is a splenocyte isolated from the spleen of a mouse immunized by an antigen derived from a 3ABC non-structural protein (NSP) of FMDV. The antigen used here is expressed by a prokaryotic cell. The monoclonal antibody produced by the hybridoma cell line CmA40 as deposited under American Type Culture Collection patent deposit number PTA-11304 can specifically recognize a 3ABC polypeptide and does not cross-react with an antiserum of swine vesicular disease virus.

Abstract: The present invention provides a rapid RT-PCR detection method and a diagnostic kit for differentiating field isolates of classical swine fever virus (CSFV) from lapinized CSF vaccine viruses in samples. In order to detecting different genotypes of CSF virus, this invention uses a pair (or pairs) of CSF virus specific primers designed from the conserved sequences within the 3?-untranslated region of CSFV which contains an insertion of 12˜13 nucleotides (poly T) in the region of the lapinized CSF vaccine virus, in comparison with the corresponding region of field isolates of CSFV. By the RT-PCR or the RT-PCR followed by a nest-PCR, field isolates of CSFV and lapinized CSF vaccine viruses in samples could be detected directly and quickly and/or differentiated in electrophoresis without further enzymatic digestion or DNA sequencing.

Abstract: A simultaneous detection, differentiation and typing system of Newcastle disease and avian influenza viruses is provided. The present invention provides a system, including an oligonucleotide microarray, avian virus specific probes is disposed on the oligonucleotide microarray and the avian viruses include Newcastle disease and avian influenza viruses, and avian virus nucleic acid products, hybridized with the avian virus specific probes on the oligonucleotide microarray. The present invention describes a fast, simultaneous and inexpensive approach to the detection of Newcastle disease virus (NDV) and avian influenza virus (AIV) and possesses good sensitivity and specificity among divergent viruses. The hybridization results on microarrays were clearly identified with the naked eyes, with no further imaging equipment needed. The present invention provides potential for rapid surveillance and differential diagnosis of these two important zoonoses in both wild and domestic birds.