Patents Assigned to Anti-Gene Development Group
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Patent number: 5405938Abstract: The present invention describes a polymer composition effective to bind, in a sequence-specific manner to a target sequence of a duplex polynucleotide, at least two different-oriented Watson/Crick base-pairs at selected positions in the target sequence. The composition includes an uncharged backbone with 5- or 6-membered cyclic backbone structures and selected bases attached to the backbone structures effective to hydrogen bond specifically with different oriented base-pairs in the target sequence. Also disclosed are subunits useful for the construction of the polymer composition. The present invention further includes methods for (i) coupling a first free or polymer-terminal subunit, and (ii) isolating, from a liquid sample, a target duplex nucleic acid fragment having a selected sequence of base-pairs.Type: GrantFiled: November 23, 1992Date of Patent: April 11, 1995Assignee: Anti-Gene Development GroupInventors: James E. Summerton, Dwight D. Weller
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Patent number: 5235033Abstract: Alpha-morpholino subunits and polymer compositions composed of alpha-morpholino subunits are disclosed. These subunits can be linked together by uncharged linkages, one to three atoms in length, joining the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide. The polymers of the present invention can be used in place of standard RNA or DNA oligomers for a number of standard laboratory procedures, for example, as probes in solid-phase systems and nucleic acid hybridization analyses. Further, the polymers of the present invention have potential use as therapeutic agents in anti-sense-type technologies.Type: GrantFiled: December 20, 1989Date of Patent: August 10, 1993Assignee: Anti-Gene Development GroupInventors: James E. Summerton, Dwight D. Weller, Eugene P. Stirchak
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Patent number: 5217866Abstract: A diagnostic reagent and system for determination of a single-stranded polynucleotide analyte having a selected target sequence. The reagent includes a solid support and multiple support-bound polymers designed to bind specifically to the analyte. Each polymer is composed of a sequence of base-complementary recognition moieties which can bind specifically to corresponding contiguous bases in the analyte target sequence, and an unbranched, substantially uncharged, substantially stereoregular backbone. A reporter in the system contains (a) a polycationic tail effective to bind electrostatically to the analyte, under conditions in which the reporter does not bind to the substantially uncharged polymers, and (b) reporter groups by which the presence of the reporter can be detected.Type: GrantFiled: December 18, 1986Date of Patent: June 8, 1993Assignee: Anti-Gene Development GroupInventors: James Summerton, Dwight Weller
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Patent number: 5166315Abstract: A polymer composition effective to bind in a sequence-specific manner to a target sequence of a duplex polynucleotide containing different Watson-Crick basepair oreientaions. The composition includes an uncharged backbone with 5- or 6-membered cyclic backbone structures and selected bases attached to the backbone structures effective to hydrogen bond specifically with different oriented basepairs in the target sequence.Type: GrantFiled: June 20, 1991Date of Patent: November 24, 1992Assignee: Anti-Gene Development GroupInventors: James E. Summerton, Dwight D. Weller
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Patent number: 5142047Abstract: A composition of polymer molecules effective to bind, with substantially uniform binding affinity, to a single-stranded polynucleotide containing a target sequence of bases. The polymer molecules are composed of a sequence of base-pairing moieties effective to hydrogen bond to corresponding, complementary bases in the target sequence, under selected binding conditions, and a predominantly uncharged, achiral backbone supporting the base-pairing moieties at positions and in orientations which allow hydrogen bonding between the pairing moieties of the polymer and the corresponding complementary bases in the target sequence. The composition has diagnostic uses, in a solid-support assay system, and therapeutic uses involving inhibition or inactivation of target polynucleotides.Type: GrantFiled: September 23, 1987Date of Patent: August 25, 1992Assignee: Anti-Gene Development GroupInventors: James Summerton, Dwight Weller, Eugene Stirchak
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Patent number: 5034506Abstract: A polymer composition is disclosed composed of morpholino subunit structures which are linked together by uncharged, achiral linkages. These linkages are one to three atoms in length, joining the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide.Type: GrantFiled: December 20, 1989Date of Patent: July 23, 1991Assignee: Anti-Gene Development GroupInventors: James E. Summerton, Dwight D. Weller