Patents Assigned to AntiCancer, Inc.
  • Patent number: 6426194
    Abstract: Enzymatic methods to determine the concentration of pyridoxal 5′-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme for homocysteinase and related enzymes and measuring the products of the reaction preferably spectrophotometrically. The invention also includes improvements in sensitivity of assays for measuring hydrogen sulfide production by measuring fluorescence as opposed to absorbance of the oxidized product of H2S with N,N-dialkyl p-phenylene diamine.
    Type: Grant
    Filed: February 1, 2000
    Date of Patent: July 30, 2002
    Assignee: AntiCancer, Inc.
    Inventors: Mingxu Xu, Qinghong Han, Yuying Tan
  • Patent number: 6372489
    Abstract: A composition and method for treating disorders related to tyrosinase gene expression and melanin biosynthesis is disclosed. The composition comprises a tyrosinase encoding nucleotide sequence and an ORF-438 encoding nucleotide sequence derived from Streptomyces, adapted for expression in mammalian cells. Also disclosed is a model system for evaluating agents that affect pigmentation.
    Type: Grant
    Filed: October 27, 1999
    Date of Patent: April 16, 2002
    Assignee: AntiCancer, Inc.
    Inventor: Ming Zhao
  • Patent number: 6329162
    Abstract: A method to assess the level of folate in a biological sample comprises: providing said sample with glycine N-methyltransferase (GMT) and with an excess of S-adenosyl methionine (SAM) and of glycine; providing a control which contains no folate with said GMT and excess SAM and glycine in comparable amounts to those provided to the sample; and comparing the concentration of at least one product formed in the sample with the concentrations of said product formed in the control, whereby the difference in levels of said product in the sample as compared to the control is directly proportional to the level of folate in the sample.
    Type: Grant
    Filed: April 17, 2000
    Date of Patent: December 11, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Qinghong Han, Li Tang, Mingxu Xu, Yuying Tan, Shigeo Yagi
  • Patent number: 6261596
    Abstract: The present invention describes a method for targeted and specific delivery of beneficial compounds, including hair dyes, melanin, proteins, and nucleic acids for gene therapy, to hair follicle cells using liposomes encapsulating the beneficial compound. Particularly preferred methods describe delivery of hair dyes, melanin or tyrosinase to the hair follicle for the purpose of improving hair color or condition, the delivery of compounds which prevent alopecia or stimulate hair growth, either by encapsulating a compound in liposomes, or by encapsulating a nucleic acid capable of expressing a protein in liposomes. Also described are liposome compositions for practicing the methods.
    Type: Grant
    Filed: May 21, 1999
    Date of Patent: July 17, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Lingna Li, Valeryi Lishko
  • Patent number: 6251384
    Abstract: A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is disclosed. The fluorescence can also be monitored by observing the tissues in situ. Vertebrate subjects which contain GFP producing tumors are useful models to study the mechanism of metastasis, as well as to evaluate candidate protocols and drugs. In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.
    Type: Grant
    Filed: January 7, 1999
    Date of Patent: June 26, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Takashi Chishima
  • Patent number: 6235968
    Abstract: A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is disclosed. The fluorescence can also be monitored by observing the tissues in situ. Vertebrate subjects which contain GFP producing tumors are useful models to study the mechanism of metastasis. In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.
    Type: Grant
    Filed: April 28, 1998
    Date of Patent: May 22, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Takashi Chishima
  • Patent number: 6235967
    Abstract: A method to follow the progression of metastasis of a primary tumor, which method comprises optionally removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the tissues in situ or observing the excised tissues for the presence of fluorescence is disclosed. Vertebrate subjects which contain GFP producing tumors are useful models to study the mechanism of metastasis. In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.
    Type: Grant
    Filed: March 27, 1998
    Date of Patent: May 22, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Takashi Chishima
  • Patent number: 6232523
    Abstract: A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is disclosed. Vertebrate subjects which contain GFP producing tumors are useful models to study the mechanism of metastasis.
    Type: Grant
    Filed: April 28, 1997
    Date of Patent: May 15, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Takashi Chishima
  • Patent number: 6231854
    Abstract: The present invention discloses the cloning of a methioninase-encoding nucleic acid molecule from Pseudomonas putida and the construction of high-level expression modules containing the methioninase-encoding nucleic acid molecule. The invention further provides expression modules that use the T7 RNA polymerase promoter to express the isolated methioninase-encoding nucleic acid molecules. Expression modules employing the T7 promoter were found to produce unexpectedly high levels of methioninase. The present invention further provides purification methods to obtain highly pure, endotoxin free methioninase, chemically modified forms of methioninase, crystallized methioninase and lyophilized methioninase preparations. The present invention further provides therapeutical methods using the disclosed recombinant methioninase preparations.
    Type: Grant
    Filed: March 2, 1999
    Date of Patent: May 15, 2001
    Assignee: Anticancer, Inc.
    Inventor: Tan Yuying
  • Patent number: 6224901
    Abstract: The present invention is directed to introduce a replacement pigment into the hair shaft through the hair follicle using a formulation of a replacement pigment in a liposomal composition.
    Type: Grant
    Filed: May 20, 1997
    Date of Patent: May 1, 2001
    Assignee: AntiCancer, Inc.
    Inventors: Lingna Li, Valervi Lishko
  • Patent number: 6140102
    Abstract: Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aformentioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction.
    Type: Grant
    Filed: November 19, 1997
    Date of Patent: October 31, 2000
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Marcin Lenz
  • Patent number: 6080127
    Abstract: The present invention provides a methods and apparatuses for use in providing rapid, selective and effective targeted delivery of beneficial substances into hair follicles. The invention relies on the use of vibrational method of delivery of substances of different size from small molecules up to particles compatible with the diameter of hair strand into the hair bulb. The skin is subjected to the vibration of about 1 Hz to about 100 Hz frequency and 0.1 mm to about 10 mm amplitude, an amount which is sufficient to cause mechanical shifts in different directions of skin structure without damage. This results in follicle effective uptake of the tested substances such as carbon particles of different size as well as melanin. The methods and apparatuses of the present invention are particularly useful in conjunction with photosensitive agents and electromagnetic irradiation. Such a combination is used to target the delivery of the agent to within hair follicles and then subsequently activate the agent.
    Type: Grant
    Filed: October 29, 1998
    Date of Patent: June 27, 2000
    Assignee: AntiCancer, Inc.
    Inventors: Lingna Li, Eugene Baranov
  • Patent number: 6066467
    Abstract: Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aforementioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction.
    Type: Grant
    Filed: June 28, 1999
    Date of Patent: May 23, 2000
    Assignee: AntiCancer, Inc.
    Inventors: Mingxu Xu, Yuying Tan, Qinghong Han, Li Tang
  • Patent number: 5998191
    Abstract: Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aformentioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction.
    Type: Grant
    Filed: July 24, 1998
    Date of Patent: December 7, 1999
    Assignee: Anticancer Inc.
    Inventors: Yuying Tan, Martin Lenz
  • Patent number: 5985540
    Abstract: Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aformentioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction.
    Type: Grant
    Filed: April 17, 1998
    Date of Patent: November 16, 1999
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Martin Lenz
  • Patent number: 5965157
    Abstract: The present invention provides a method to specifically target hair follicles with formulations which effect the production of hair coloring pigments in the follicle. Liposomal formulations for this purpose are disclosed.
    Type: Grant
    Filed: May 20, 1997
    Date of Patent: October 12, 1999
    Assignee: Anticancer Inc.
    Inventors: Lingna Li, Valeryi Lishko
  • Patent number: 5914126
    Abstract: The invention provides methods to deliver macromolecules to hair follicles selectively using formulations of these macromolecules in liposomal separations.
    Type: Grant
    Filed: May 20, 1997
    Date of Patent: June 22, 1999
    Assignee: AntiCancer, Inc.
    Inventors: Lingna Li, Valeryi Lishko
  • Patent number: 5891704
    Abstract: The present invention discloses the cloning of a methioninase-encoding nucleic acid molecule from Pseudomonas putida and the construction of high-level expression modules containing the methioninase-encoding nucleic acid molecule. The invention further provides expression modules that use the T7 RNA polymerase promoter to express the isolated methioninase-encoding nucleic acid molecules. Expression modules employing the T7 promoter were found to produce unexpectedly high levels of methioninase. The present invention further provides purification methods to obtain highly pure, endotoxin free methioninase, chemically modified forms of methioninase, crystallized methioninase and lyophilized methioninase preparations. The present invention further provides therapeutical methods using the disclosed recombinant methioninase preparations.
    Type: Grant
    Filed: May 3, 1996
    Date of Patent: April 6, 1999
    Assignee: AntiCancer, Inc.
    Inventor: Tan Yuying
  • Patent number: 5888506
    Abstract: Improved formulations of methioninase which contain methioninase in crystalline, lyophilized, or conjugated form are disclosed. The methioninase in these compositions has a specific activity of at least 20 U/mg and the formulations are detergent free and contain less than 1 ng endotoxin per mg protein.
    Type: Grant
    Filed: August 19, 1997
    Date of Patent: March 30, 1999
    Assignee: AntiCancer, Inc.
    Inventor: Yuying Tan
  • Patent number: 5849579
    Abstract: Methods and compositions for a native-state histoculturing system for skin are disclosed. Skin samples are placed on an extracellular support matrix immersed in a medium whereby the internal surface of the skin is adjacent to the matrix and the external surface is exposed above the surface of the medium and the skin maintained in the medium under skin culturing conditions. An extracellular support matrix comprising a collagen-containing gel and homopolysaccharide sponge or a combination matrix of a top layer of a collagen-containing gel and a bottom layer of a homopolysaccharide sponge is also disclosed. Skin toxicity, hair growth, anti-aging and anti-wrinkling assays utilizing the histoculturing system of the present invention are also disclosed.
    Type: Grant
    Filed: May 3, 1996
    Date of Patent: December 15, 1998
    Assignee: Anticancer, Inc.
    Inventor: Lingna Li