Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.
Type:
Grant
Filed:
December 11, 2007
Date of Patent:
February 12, 2013
Assignee:
APDN (B.V.I.), Inc.
Inventors:
Thomas Kwok, Ming-Hwa Liang, Stephane Shu Kin So
Abstract: A method to link a light emitting reporter to biomolecules with nucleotide oligomers is described. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particle generated by the methods of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.
Type:
Grant
Filed:
December 11, 2007
Date of Patent:
February 28, 2012
Assignee:
APDN, Inc.
Inventors:
Thomas Kwok, Ming-Hwa Liang, Stephane Shu Kin So