Patents Assigned to APDN (B.V.I.), Inc.
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Publication number: 20220136036Abstract: The invention relates to methods of treating diseases comprising administering to a subject a composition comprising the recombinant adeno-associated virus (rAAV). The rAAV is produced by a method comprising: obtaining a template DNA sequence containing a [ITR-cargo-ITR] DNA sequence motif; designing a PCR primer pair such that the 3? terminus of both the forward and reverse PCR primers overlap only about the last 2-8 bases of the A/A? ITR sequences and the 5? terminus of both the forward and reverse PCR primers extend into about 20-35 bases of the flanking sequences; performing PCR with cycling parameters comprising a combined annealing/extension step at a temperature greater than 70° C., thereby producing a plurality of amplicon polynucleotides containing the desired [ITR-cargo-ITR] DNA sequence motif; transfecting the amplicon polynucleotides containing the desired [ITR-cargo-ITR] DNA sequence motif into a packaging cell line; and purifying the lysed cells to collect a quantity of rAAV.Type: ApplicationFiled: January 18, 2022Publication date: May 5, 2022Applicant: APDN (B.V.I) Inc.Inventors: Michael E. HOGAN, Stephen HUGHES, Yuhua SUN
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Patent number: 10995371Abstract: Methods of incorporating coded DNA into elastomeric materials and compositions thereof are claimed. Methods of recovering information from elastomeric materials with coded DNA and authenticating silicone objects with coded DNA are also claimed.Type: GrantFiled: October 2, 2017Date of Patent: May 4, 2021Assignee: APDN (B.V.I.) Inc.Inventors: Michael Hogan, In-Young Yang, Yuhua Sun, Lawrence Jung
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Patent number: 10920274Abstract: A composition comprising submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.Type: GrantFiled: February 7, 2018Date of Patent: February 16, 2021Assignee: APDN (B.V.I.) Inc.Inventors: Michael E. Hogan, Lawrence Jung, Nicole Richter, Yuhua Sun, Maciej Szczepanik
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Patent number: 10760182Abstract: A method of marking fibers, wherein the method includes providing a plurality of fibers; depositing a marker onto at least a portion of the fibers, the depositing being performed with a delivery mechanism comprising one or more outlets; and thereby marking the fibers. Also provided is a device for marking fibers, including a transport system adapted to transport fibers in a direction of a marker delivery apparatus positioned along the transport system; the delivery apparatus includes one or more outlets, adapted to deposit a solution of the marker through the outlets onto at least a portion of the fibers; and thereby marking the fibers. Authentication of a fibrous material using the marking method of the invention followed obtaining a sample of the marked fibers and assaying the sample for the presence of the nucleic acid marker; and thereby determining whether the fibrous material is authentic or counterfeit.Type: GrantFiled: December 16, 2014Date of Patent: September 1, 2020Assignee: APDN (B.V.I.) INC.Inventors: Alex Phidung Tran, Abdelkrim Berrada, MingHwa Benjamin Liang, Lawrence Jung
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Patent number: 10745825Abstract: Encrypted markers that are not readily detectable can be revealed by treatment with a specific reagent used as a developer to reveal a readily detectable physical property of the marker, such as a characteristic fluorescence emission after excitation with a particular excitation wavelength, or to reveal a visible color. The encrypted marker can be developed in situ, or a sample can be removed by brushing, scraping, swabbing or scratching the marked object or item and developing the encrypted marker or a sample thereof with the appropriate developer to reveal an overt marker or optical signal. The encrypted marker may include a DNA taggant.Type: GrantFiled: July 5, 2018Date of Patent: August 18, 2020Assignee: APDN (B.V.I.) INC.Inventors: Maciej B. Szczepanik, MingHwa Benjamin Liang
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Patent number: 10741034Abstract: A method of marking an inventory item includes providing an activatable smoke generator and a reservoir for holding a smoke fluid and adapted to provide a flow of smoke fluid to the generator. The reservoir contains a smoke fluid including a carrier nucleic acid having a uniquely identifiable sequence, and upon activation of the smoke generator, marker smoke is generated and targeted to flow over the inventory item. The method further includes activating the smoke generator to produce the marker smoke including the carrier nucleic acid so as to cause the marker smoke to flow over the inventory item and thereby to detectably mark the inventory item with carrier nucleic acid. The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate.Type: GrantFiled: December 15, 2014Date of Patent: August 11, 2020Assignee: APDN (B.V.I.) INC.Inventors: Abdelkrim Berrada, MingHwa Benjamin Liang, Lawrence Jung
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Patent number: 10519605Abstract: Methods for marking cellulosic products, including cellulosic fibers such as lyocell and cellulosic films, including methods for marking such products with a detectable nucleic acid marker to identify and validate the origin or authenticity of the products or items manufactured using such products. Detectably-marked cellulosic products marked with nucleic acid markers for authentication, validation and tracking are also provided.Type: GrantFiled: March 22, 2017Date of Patent: December 31, 2019Assignee: APDN (B.V.I.), Inc.Inventors: Lawrence Jung, Michael E. Hogan, Ming Hwa Benjamin Liang
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Publication number: 20190241982Abstract: The invention provides for a system to track the origin of cannabis products and cannabis derivative products without the need for packaging or labeling through the use of nucleic acid tags. The invention further provides for a method of tracking the origin of cannabis products and cannabis derivative products via the application of nucleic acid tags to cannabis plants.Type: ApplicationFiled: February 1, 2019Publication date: August 8, 2019Applicant: APDN (B.V.I.) Inc.Inventors: Michael E. HOGAN, Gordon HOPE, James A. HAYWARD, John SHERMAN, Thomas ACKERMAN, Brian VISCOUNT
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Publication number: 20180312998Abstract: Encrypted markers that are not readily detectable can be revealed by treatment with a specific reagent used as a developer to reveal a readily detectable physical property of the marker, such as a characteristic fluorescence emission after excitation with a particular excitation wavelength, or to reveal a visible color. The encrypted marker can be developed in situ, or a sample can be removed by brushing, scraping, swabbing or scratching the marked object or item and developing the encrypted marker or a sample thereof with the appropriate developer to reveal an overt marker or optical signal. The encrypted marker may include a DNA taggant.Type: ApplicationFiled: July 5, 2018Publication date: November 1, 2018Applicant: APDN (B.V.I.) INC.Inventors: Maciej B. SZCZEPANIK, MingHwa Benjamin LIANG
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Patent number: 10059981Abstract: The invention provides methods for increasing the recoverability of taggants from an object. The methods include the steps of incorporating a taggant into a solution; mixing the solution including the taggant with a perturbant to form a first perturbant taggant solution; mixing the first perturbant taggant solution with a polymer to form a second perturbant taggant polymer solution; and applying the second perturbant taggant polymer solution to at least a portion of the object to form a taggant-coated object. Methods for authentication of a taggant marked object are also provided.Type: GrantFiled: March 24, 2016Date of Patent: August 28, 2018Assignee: APDN (B.V.I.) INC.Inventors: Lawrence Jung, MingHwa Benjamin Liang, James A. Hayward
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Patent number: 10047282Abstract: Encrypted markers that are not readily detectable can be revealed by treatment with a specific reagent used as a developer to reveal a readily detectable physical property of the marker, such as a characteristic fluorescence emission after excitation with a particular excitation wavelength, or to reveal a visible color. The encrypted marker can be developed in situ, or a sample can be removed by brushing, scraping, swabbing or scratching the marked object or item and developing the encrypted marker or a sample thereof with the appropriate developer to reveal an overt marker or optical signal. The marker can be revealed by exposure of the encrypted marker or a sample thereof to the developer in any suitable form, such as a solution, a slurry, a swab, a solid (such as in granular form), or a gas or a vapor that includes a developer.Type: GrantFiled: March 18, 2015Date of Patent: August 14, 2018Assignee: APDN (B.V.I.) INC.Inventors: Maciej B. Szczepanik, MingHwa Benjamin Liang
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Patent number: 9963740Abstract: Provided are a method and device for marking an article for security, tracking or authentication. The method includes depositing a solution comprising a nucleic acid marker onto at least a portion of the article. The nucleic acid marker may be activated, for example, by adding a functional group to the nucleic acid marker. The activation of the nucleic acid marker may be performed by exposure to alkaline conditions. The method is well suited for marking fibers and textiles, as well as many other items.Type: GrantFiled: September 26, 2014Date of Patent: May 8, 2018Assignee: APDN (B.V.I.), INC.Inventors: Abdelkrim Berrada, MingHwa Benjamin Liang, Lawrence Jung
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Patent number: 9938586Abstract: Methods for distinguishing between cotton species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the species of cotton utilized in the textile or cotton material of interest.Type: GrantFiled: February 27, 2014Date of Patent: April 10, 2018Assignee: APDN (B.V.I.) INC.Inventors: MingHwa Benjamin Liang, Stephane Shu Kin So
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Patent number: 9919512Abstract: The invention provides a method of marking an item with naturally-derived or synthetic non-natural polymeric marker molecules, such as a DNA or Peptide marker in conjunction with optional visible or rapid scan reporters for authenticating or tracking, in which the method includes providing an item for marking, and applying a medium including a DNA marker to the item. The invention further provides methods for stably binding and immobilizing activated deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. Also provided are methods for increasing the recoverability of taggants from an object.Type: GrantFiled: February 22, 2016Date of Patent: March 20, 2018Assignee: APDN (B.V.I.) INC.Inventors: Lawrence Jung, James A. Hayward, MingHwa Benjamin Liang, Abdelkrim Berrada
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Patent number: 9904734Abstract: A multi-mode reader instrument capable of detecting and determining digital data from a signal from one or more markers, indicia or taggants on an object, the markers, indicia or taggants such as a bar code, a QR code, an RFID, an optical compound, a fluorescent compound, a phosphorescent compound, a DNA taggant, an upconverting phosphor (UCP), a chemical dye, a digitized image, a radioactive compound, an olfactory compound or a thermal attribute of the object is provided. Also, provided is a method and a system for identifying an object, the system includes: a multi-mode reader instrument for detecting data from a signal from one or more markers, indicia or taggants on an object and assignment of digital code for the marker and a database for securing and retrieving information relevant to the item.Type: GrantFiled: October 7, 2014Date of Patent: February 27, 2018Assignee: APDN (B.V.I.) INC.Inventors: Judith Murrah, Phidung H. Tran, Maciej B. Szczepanik, MingHwa Benjamin Liang, James A. Hayward
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Patent number: 9790538Abstract: The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. The alkaline conditions are produced by mixing the deoxyribonucleic acid with an alkaline solution having a pH of about 9.0 or higher, and contacting the deoxyribonucleic acid to the substrate. The immobilized DNA can be used as a taggant and can be used in combination with other detectable taggants, such as optical reporters. Methods for authentication of a DNA marked object are also provided.Type: GrantFiled: March 7, 2013Date of Patent: October 17, 2017Assignee: APDN (B.V.I.) INC.Inventors: Abdelkrim Berrada, MingHwa Benjamin Liang, Lawrence Jung, Kurt Jensen
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Publication number: 20170228826Abstract: A method and system of identifying articles in commerce including tagging a raw material during said raw material's manufacturing process with a unique molecular marker in a home country in which the raw material was produced. The marker identifies home country information. The tagged raw material is transported from the home country to a foreign country. The tagged raw material is processed in the foreign country to generate a processed product. The processed product is transported to the home country. The processed product is analyzing to determine the presence of the marker in the processed product. A determination is made whether the raw material originated in the home country in response to the detection of the marker.Type: ApplicationFiled: February 8, 2017Publication date: August 10, 2017Applicant: APDN (B.V.I.) Inc.Inventors: James A. Hayward, MeiLin Wan
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Publication number: 20160264687Abstract: A method of binding a detectable marker to a surface comprising the steps of exposing the surface to an electron beam to produce an electron beam-treated surface; applying a detectable marker to the electron beam-treated surface; and thereby producing a surface-bound detectable marker on the treated surface. The detectable marker can be any suitable marker such as an optical marker, a dye, a fluorophore, a biomolecule, a metal, or a rare earth element. Also provided is a composition including a detectable marker immobilized on a surface pre-treated with an electron beam.Type: ApplicationFiled: October 31, 2014Publication date: September 15, 2016Applicant: APDN (B.V.I.) Inc.Inventor: Phidung H. TRAN
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Patent number: 9412284Abstract: A method of encrypting information includes converting an information sequence represented as a strings of bits into a curvilinear coding system, where each element of the curvilinear coding system corresponds to a sub-sequence of successive same-valued bits, converting said curvilinear coding system into a at least one closed curve, and embedding said at least one closed curve in a 2-dimensional pattern. A method for decrypting information includes extracting at least one closed curve from a digitized 2-dimensional pattern, and converting the at least one closed curve into an information sequence represented as a string of bits.Type: GrantFiled: April 15, 2014Date of Patent: August 9, 2016Assignee: APDN (B.V.I.) INC.Inventors: Phidung H. Tran, Minghwa Benjamin Liang, Lawrence Jung, James A. Hayward
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Publication number: 20160168781Abstract: A method of marking fibers, wherein the method includes providing a plurality of fibers; depositing a marker onto at least a portion of the fibers, the depositing being performed with a delivery mechanism comprising one or more outlets; and thereby marking the fibers. Also provided is a device for marking fibers, including a transport system adapted to transport fibers in a direction of a marker delivery apparatus positioned along the transport system; the delivery apparatus includes one or more outlets, adapted to deposit a solution of the marker through the outlets onto at least a portion of the fibers; and thereby marking the fibers. Authentication of a fibrous material using the marking method of the invention followed obtaining a sample of the marked fibers and assaying the sample for the presence of the nucleic acid marker; and thereby determining whether the fibrous material is authentic or counterfeit.Type: ApplicationFiled: December 16, 2014Publication date: June 16, 2016Applicant: APDN (B.V.I.) INC.Inventors: Alex Phidung TRAN, Abdelkrim BERRADA, MingHwa Benjamin LIANG, Lawrence JUNG