Abstract: The present invention relates to an improved method of formation of disulfide bridges in substances bearing SH groups, in particular peptides, for example by formation of intramolecular disulfide bridges, in which a heterocyclic compound having at least one nitrogen atom (e.g. caffeine or a caffeine-like substance) is used for catalysis of the reaction. It was found, surprisingly, that addition of the heterocyclic substance increases both the yield and the purity of the product bearing disulfide bridges.
Type:
Application
Filed:
December 21, 2007
Publication date:
August 12, 2010
Applicant:
APLAGEN GMBH
Inventors:
Karsten Knorr, Marco Emgenbroich, Carsten Büngener
Abstract: Use of an activated solid phase and a peptide-conjugated anchoring part for solid phase peptide synthesis, wherein the anchoring part is coordinatively and reversibly attached to the activated solid phase.
Abstract: Peptides, methods for the preparation thereof, medicaments containing the peptides, and their use in selected indications, such as the treatment of various forms of anemia and stroke.
Type:
Grant
Filed:
January 25, 2005
Date of Patent:
September 15, 2009
Assignee:
Aplagen GmbH
Inventors:
Hans-Georg Frank, Franz-Peter Bracht, Udo Haberl, Andreas Rybka
Abstract: The present invention relates to an improved method of formation of disulfide bridges in substances bearing SH groups, in particular peptides, for example by formation of intramolecular disulfide bridges, in which a heterocyclic compound having at least one nitrogen atom (e.g. caffeine or a caffeine-like substance) is used for catalysis of the reaction. It was found, surprisingly, that addition of the heterocyclic substance increases both the yield and the purity of the product bearing disulfide bridges.
Type:
Application
Filed:
December 23, 2006
Publication date:
March 26, 2009
Applicant:
AplaGen GmbH
Inventors:
Karsten Knorr, Marco Emgenbroich, Carsten Bungener
Abstract: A method of assessing the titre of fertility-relevant autoantibodies of a mammalian female, wherein said method comprises: the presentation of a defined single molecule capable of binding to the idiotype of the fertility-relevant autoantibody, and determining the amount of autoantibody being bound by said molecule from a sample of a body fluid of said mammalian female.
Type:
Application
Filed:
February 21, 2005
Publication date:
October 25, 2007
Applicant:
AplaGen GmbH
Inventors:
Hans-Georg Frank, Andreas Rybka, Andrea Borges, Alexandra Greindl
Abstract: The present invention therefore presents modules from which helical constraints can be built by very flexible strategies. The peptide bonds involved partially compensate the hydrophobic nature of the disulfide bonds, which are also included into the constraint strategy. Thus, the invention presents solutions, by means of which peptide bonds or closure of disulfide bridges can be used alternatively for closure of the constraint. This offers greater synthetic flexibility. Moreover, the peptide bonds are more hydrophilic than disulfide bridges alone and offer the advantage of better solubility of the product in an aqueous surrounding. It is possible to attach solvation tags like glycosyl moieties, polyethylenglycol or other suitable extensions or appendices to the helical constraint structure. Usually, such a hydrophilic helical constraint structure replaces two hydrophobic amino acid side chains and thus improves pharmacologic properties of the molecule.
Type:
Application
Filed:
October 18, 2004
Publication date:
September 6, 2007
Applicant:
APLAGEN GMBH
Inventors:
Hans-Georg Frank, Udo Haberl, Franzpeter Bracht, Andreas Rybka
Abstract: Use of an activated solid phase and an anchoring part which is attached to a peptide for solid phase peptide synthesis, wherein the anchoring part is coordinatively and reversibly attached to the activated solid phase. Furthermore provided is a process for competitively detachment of said anchoring part, for purification and refolding of said peptides. Provided are peptides with an anchoring part for coordinative and reversible attachment of said peptides to an activated solid phase.
Type:
Application
Filed:
May 24, 2004
Publication date:
February 1, 2007
Applicant:
AplaGen GmbH
Inventors:
Andreas Rybka, Hans-Georg Frank, Franz-Peter Bracht, Udo Haberl