Abstract: This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions.
Type:
Application
Filed:
December 22, 2006
Publication date:
August 28, 2008
Applicant:
Applera Coporation
Inventors:
James M. Coull, Mark J. Fiandaca, Mark D. Kristjanson, Jens J. Hyldig-Nielsen, Theresa S. Creasey
Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
Type:
Grant
Filed:
April 14, 2004
Date of Patent:
October 31, 2006
Assignee:
Applera Coporation
Inventors:
Paul David Grossman, Steven Fung, Steven Michael Menchen, Sam Lee Woo, Emily Susan Winn-Deen
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