Abstract: A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and a biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits an amplified and detectable signal when it reacts with the specific infectious agent. Signal detection is enhanced through the release of LPS from the cells of the infectious agent.

Abstract: A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and at least one biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; wherein the biosensor emits a detectable signal when it reacts with the specific infectious agent; wherein the biosensor is a hybridoma cell that naturally expresses an endogenous anti-target antigen specific IgM; and wherein the hybridoma cell has been converted to a B cell receptor biosensor by introducing a detectable reporter gene into the cell.