Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support.

Abstract: Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.

Abstract: A matrix for mass spectrometry, the matrix comprising an additive that minimizes or eliminates adduct formation and/or chemical noise generation is disclosed. Also disclosed is a method for depositing a matrix on a MALDI target plate and a disposable plate having a dissolvable pre-matrix deposited thereon. The matrix material can be 2,5-dimethoxybenzoic acid and a monobasic or dibasic salt or tribasic salt as an adduct-reducing additive, such as ammonium monobasic phosphate and sulfate salts, dibasic citrate salts and tribasic citrate salts.

Abstract: Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.