Patents Assigned to Applied Biosystems, LLC.
  • Electrowetting Dispensing Devices and Related Methods
    Publication number: 20140251811
    Abstract: A device having a first substrate and a second substrate separated from the first substrate to form a volume between the first and second substrates, electrodes disposed on the first and second substrates and facing the volume, each substrate comprising at least one electrode, and a first insulating layer disposed on the first substrate and a second insulating layer disposed on the second substrate to separate the electrodes from the volume.
    Type: Application
    Filed: April 28, 2014
    Publication date: September 11, 2014
    Applicant: APPLIED BIOSYSTEMS LLC
    Inventors: Charles S. VANN, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
  • Sample preparation for in situ nucleic acid analysis, methods and compositions therefor
    Patent number: 8828664
    Abstract: Sample preparation processes for in situ RNA or DNA analysis, methods and compositions therefor are provided. Processes provided herein allow DNA or RNA analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or extraction. The preparation process can be carried out at room temperature in as little as seven minutes and is amenable to high throughput processing using manual or robotic platforms.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: September 9, 2014
    Assignee: Applied Biosystems, LLC.
    Inventors: Richard Fekete, Annalee Nguyen
  • Fluid Processing Device for Oligonucleotide Synthesis and Analysis
    Publication number: 20140249057
    Abstract: The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: September 4, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventor: Sergey V. Ermakov
  • Caps for Sample Wells and Microcards for Biological Materials
    Publication number: 20140248691
    Abstract: A cover for a biological sample well tray, comprising a cap for sealing a sample well. The cap comprises a well lens for focusing light into the sample well and collecting light from the sample. In another aspect, the cap comprises an elongate portion configured to permit incoming light to pass into the sample well and out of the sample well. Various other aspects comprise a microcard for biological material, and an apparatus for a plurality of sample well strips. A method for testing a biological sample is also provided.
    Type: Application
    Filed: November 22, 2013
    Publication date: September 4, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Eugene Young, Steven Boege, Donald Sandell
  • Device for amplifying target nucleic acid
    Patent number: 8822183
    Abstract: A device for amplifying target nucleic acid in a sample can include a planar fluidic assembly including a transparent substrate, a porous material layer on a surface of the transparent substrate, and a cover over the porous material layer and sealingly affixed to the substrate. The cover may be spaced from the porous material layer and a flow channel defined between the porous material layer and the cover. The flow channel may have a uniform cross-section from a first end to a second end.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: September 2, 2014
    Assignees: Applied Biosystems, LLC, The United States of America, as represented by the Secretary, Department of Health and Sciences
    Inventors: James F. Brown, Jonathan E. Silver
  • Phenyl Xanthene Dyes
    Publication number: 20140242665
    Abstract: Fluorescent phenyl xanthene dyes are described that comprise any fluorescein, rhodamine or rhodol comprising a particular C9 phenyl ring. One or both of the ortho groups on the lower C9 phenyl ring is ortho substituted with a group selected from alkyl, heteroalkyl, alkoxy, halo, haloalkyl, amino, mercapto, alkylthio, cyano, isocyano, cyanato, mercaptocyanato, nitroso, nitro, azido, sulfeno, sulfinyl, and sulfino. In one embodiment, halo and/or hydroxy groups are used. Optimal dyes contain a lower C9 phenyl ring in which both ortho groups are the same and the lower ring exhibits some form a symmetry relative to an imaginary axis running from the phenyl rings point of attachment to the remainder of the xanthene dye through a point para to the point of attachment. The phenyl xanthene dyes may be activated. Furthermore, the phenyl xanthene dyes may be conjugated to one or more substances including other dyes.
    Type: Application
    Filed: February 24, 2014
    Publication date: August 28, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Joe Y. LAM, Steven MENCHEN, Ruiming ZOU, Scott BENSON
  • Sample Chamber Array and Method for Processing a Biological Sample
    Publication number: 20140242593
    Abstract: A sample chamber array is provided. The sample chamber array may comprise at least one reservoir in fluid communication with at least one sample chamber, and a movable portion defining the sample chamber. The reservoir is fillable with a liquid biological sample. The movable portion may be movable with respect to the remainder of the sample chamber from a first position to a second position. In the first position the movable portion is concave and the sample chamber is without biological sample. In the second position the movable portion is convex and the sample chamber comprises biological sample. The movement of the movable portion to the second position causes a pressure drop to transport the biological sample into the sample chamber from the at least one reservoir. Methods for processing a biological sample and methods of making a sample chamber array are also provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: August 28, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventor: Donald R. Sandell
  • Heated Cover Methods and Technology
    Publication number: 20140238976
    Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.
    Type: Application
    Filed: February 3, 2014
    Publication date: August 28, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Kirk HIRANO, Patrick KINNEY, Douwe HAGA, Jason BABCOKE, Albert CARRILLO, H. Pin KAO, James NURSE
  • Multiplex Compositions And Methods For Quantification Of Human Nuclear DNA And Human Male DNA And Detection of PCR Inhibitors
    Publication number: 20140243225
    Abstract: The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
    Type: Application
    Filed: May 2, 2013
    Publication date: August 28, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventor: Applied Biosystems, LLC
  • Methods for multiplexing amplification reactions
    Patent number: 8815546
    Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: August 26, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: John Gerdes, Elaine Best, Jeffrey M. Marmaro
  • SIGNAL NOISE REDUCTION FOR IMAGING IN BIOLOGICAL ANALYSIS
    Publication number: 20140232910
    Abstract: A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.
    Type: Application
    Filed: January 21, 2014
    Publication date: August 21, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Austin TOMANEY, Mark OLDHAM
  • Polyelectrolyte-Coated Ion-Exchange Particles
    Publication number: 20140235846
    Abstract: A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.
    Type: Application
    Filed: February 18, 2014
    Publication date: August 21, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Michael Harrold, Aldrich Lau
  • Reverse transcription primers and methods of design
    Patent number: 8809513
    Abstract: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.
    Type: Grant
    Filed: August 22, 2012
    Date of Patent: August 19, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Xiaowei Wang, Xiaohui Wang, Robert Setterquist
  • Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
    Patent number: 8809040
    Abstract: An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: August 19, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Howard G. King, Steven J. Boege, Eugene F. Young, Mark F. Oldham
  • Poly and Copoly(N-vinylamide)s and Their Use In Capillary Electrophoresis
    Publication number: 20140209461
    Abstract: The invention relates generally to polymers and copolymers comprising N-vinylamide-type monomers, their preparation, and compositions, such as electrophoresis separation media, containing the same; to supports, such as capillaries, containing these polymers; and methods for separating a mixture of biomolecules, especially polynucleotides, using capillary electrophoresis. Separation media comprising such polymers yield advantageous performance in the analysis and separation of biomolecules by capillary electrophoresis.
    Type: Application
    Filed: January 6, 2014
    Publication date: July 31, 2014
    Applicant: Applied Biosystems, LLC
    Inventor: Aldrich N.K. LAU
  • Graft copolymers, their preparation and use in capillary electrophoresis
    Patent number: 8784627
    Abstract: The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis.
    Type: Grant
    Filed: September 23, 2009
    Date of Patent: July 22, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Aldrich N. Lau
  • Methods for the reduction of stutter in microsatellite amplification
    Patent number: 8785126
    Abstract: The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.
    Type: Grant
    Filed: September 12, 2012
    Date of Patent: July 22, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Sulekha Rao Coticone, William Bloch
  • LIGHT COLLECTION SYSTEM
    Publication number: 20140199715
    Abstract: The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source.
    Type: Application
    Filed: January 20, 2014
    Publication date: July 17, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Dar BAHATT, Konrad FAULSTICH
  • THERMUS BROCKIANUS NUCLEIC ACID POLYMERASES
    Publication number: 20140193877
    Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Application
    Filed: December 13, 2013
    Publication date: July 10, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Elena BOLCHAKOVA, James ROZZELLE
  • AXIAL ILLUMINATION FOR CAPILLARY ELECTROPHORESIS
    Publication number: 20140192532
    Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.
    Type: Application
    Filed: December 16, 2013
    Publication date: July 10, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Richard Reel, Eric Nordman