Abstract: Disclosed is a protein that specifically binds carcinoembryonic actigen (CEA) in the presence of divalent cation in vitro. This protein has a molecular weight of about 20 kD as determined by SDS-polyacrylamide gel electrophoresis, is glycosylated, and includes the amino acid sequence set forth in the Sequence Listing as SEQ ID NO:4. Also disclosed are antibodies that recognize the CEA binding protein, methods of detecting carcinoma, methods of treating carcinoma, and a kit for screening a patient for carcinoma.

Abstract: Disclosed is a protein that specifically binds carcinoembryonic actigen (CEA) in the presence of divalent cation in vitro. This protein has a molecular weight of about 21 kD as determined by SDS-polyacrylamide gel electrophoresis, is glycosylated, and includes the amino acid sequence set forth in the Sequence Listing as SEQ ID NO: 2. Also disclosed are antibodies that recognize the CEA binding protein, methods of detecting carcinoma, methods of treating carcinoma, and a kit for screening a patient for carcinoma.

Abstract: Disclosed are methods of isolating a protein which binds carcinoembryonic antigen. These methods include the following steps. A biological sample containing carcinoembryonic antigen (CEA) and a CEA-binding protein (CBP) is provided and contacted with a divalent cation at a concentration and for a time sufficient to allow the binding of the CBP to CEA, thereby forming a CBP-CEA conjugate. The sample is then contacted with an adsorbent that binds CEA for a time sufficient to allow adsorbance to the adsorbent. Portions of the sample not adsorbed are removed. The CBP is then disassociated from the conjugate and is collected.

Abstract: Monoclonal antibodies reactive with oncogenic and activated ras p21 proteins containing aspartic acid or valine at position 13 and unreactive with normal ras p21 proteins containing glycine at position 13. The antibodies are secreted by hybridomas obtained by immunizing mice with synthetic peptides corresponding in amino acid sequence to positions 5-19 or normal ras p21 proteins, except having aspartic acid or valine in place of glycine at position 13 and except for the addition of cysteine at the amino terminal end of the peptide. The antibodies and immunoreactive fragments are useful in the diagnosis, staging and classification of malignant and premalignant lesions.

Abstract: Pump apparatus (10; 110) for the controlled ingress and egress of a fluid comprising, a generally cylindrical housing (11; 111), flexible septum (55; 155) disposed within the housing and attached to the housing substantially axially medially thereof, an electromagnet assembly (65; 165, 165') affixed to the septum, a pair of inlet ports (25, 25; 125, 125) in the housing with one disposed to either side of the septum, valves (35; 135) in the inlet ports permitting only ingress of fluid to said housing, a pair of outlet ports (26, 26; 126, 126) in the housing with one disposed to either side of the septum, valves (35; 135) in the outlet ports permitting only the egress of fluid from the housing, permanent magnets (45, 46; 145, 146) disposed proximate the axial extremities of the housing, and a controller (70; 170) for selectively energizing the electromagnet assembly for the controlled displacement of the septum axially of the housing to alternately effect the ingress and egress of fluid from the inlet and outle