Patents Assigned to Applied Biotechnology Institute
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Patent number: 11124799Abstract: Vaccines, methods of producing, and methods of using are provided in which a protective response to Valley Fever disease is produced when administered to an animal. The vaccine provides for expression of Coccidioides sp. Ag2 polypeptide in a plant or plant part, linked to a promoter preferentially directing expression to seed tissue of the plant or plant part. Further embodiments provide the polypeptide is targeted to the cell wall, vacuole or endoplasmic reticulum. The polypeptide may be fused to a dendritic cell targeting dendritic cell or a heat labile enterotoxin. Increased expression levels in the plant or plant part are obtained. The vaccine comprising the plant-produced Ag2 polypeptide may be a glucan chitin particle comprising the Ag2 polypeptide. The plant or plant materials in an embodiment may be orally administered.Type: GrantFiled: June 12, 2019Date of Patent: September 21, 2021Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Celine Hayden
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Patent number: 11090380Abstract: Methods of vaccination of animals are provided. In an embodiment, a paired administration of a primer vaccine provides for non-oral administration of a vaccine and an oral administration of the vaccine and can be followed by a paired administration of a booster vaccine of a non-oral administration and an oral administration. Embodiments provide the non-oral and oral administration are within three days of each other. The methods provide for improved protective response in an animal compared to administration of a primer non-oral administered vaccine followed by three booster non-oral administered vaccines. An adjuvant is provided that is a microcrystalline polysaccharide-based adjuvant derived from delta inulin.Type: GrantFiled: May 28, 2019Date of Patent: August 17, 2021Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Celine Hayden
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Patent number: 10308948Abstract: A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.Type: GrantFiled: October 18, 2012Date of Patent: June 4, 2019Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Erin Egelkrout, Celine Hayden
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Patent number: 10150970Abstract: Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.Type: GrantFiled: March 6, 2014Date of Patent: December 11, 2018Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Celine Hayden, Rafael Jimenez-Flores
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Patent number: 9944937Abstract: An enhanced globulin-1 regulatory region is shown, a nucleotide sequence of which includes at least one additional copy of a region of the globulin-1 regulatory region which includes at least one transcriptional factor binding domain, combined with a transcription initiation site and translation start site. The promoter provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant.Type: GrantFiled: July 26, 2012Date of Patent: April 17, 2018Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Celine Hayden
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Patent number: 9441232Abstract: A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly pericarp preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the pericarp tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the pericarp of monocotyledonous plants, particularly cereals, and maize.Type: GrantFiled: March 7, 2013Date of Patent: September 13, 2016Assignee: Applied Biotechnology Institute, IncInventors: John Howard, Erin Engelkrout
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Publication number: 20140205625Abstract: Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.Type: ApplicationFiled: March 6, 2014Publication date: July 24, 2014Applicant: APPLIED BIOTECHNOLOGY INSTITUTE, INC.Inventors: John Howard, Celine Hayden, Rafael Jimenez-Flores
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Patent number: 8709742Abstract: Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional ?-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.Type: GrantFiled: October 9, 2010Date of Patent: April 29, 2014Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Gina Fake
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Patent number: 8709761Abstract: Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional ?-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.Type: GrantFiled: February 11, 2011Date of Patent: April 29, 2014Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Gina Fake
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Patent number: 8642749Abstract: A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.Type: GrantFiled: October 9, 2010Date of Patent: February 4, 2014Assignee: Applied Biotechnology InstituteInventors: Stephen J Streatfield, Robert Love, Jeff Bray
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Publication number: 20130305413Abstract: A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly pericarp preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the pericarp tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the pericarp of monocotyledonous plants, particularly cereals, and maize.Type: ApplicationFiled: March 7, 2013Publication date: November 14, 2013Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: John Howard, Erin Engelkrout
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Patent number: 8558058Abstract: Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.Type: GrantFiled: September 2, 2005Date of Patent: October 15, 2013Assignee: Applied Biotechnology InstituteInventors: Elizabeth Hood, John Howard
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Publication number: 20130042370Abstract: A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.Type: ApplicationFiled: October 18, 2012Publication date: February 14, 2013Applicant: APPLIED BIOTECHNOLOGY INSTITUTE, INC.Inventor: Applied Biotechnology Institute, Inc.
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Publication number: 20130031664Abstract: An enhanced globulin-1 regulatory region is shown, a nucleotide sequence of which includes at least one additional copy of a region of the globulin-1 regulatory region which includes at least one transcriptional factor binding domain, combined with a transcription initiation site and translation start site. The promoter provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant.Type: ApplicationFiled: July 26, 2012Publication date: January 31, 2013Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: John Howard, Celine Hayden
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Publication number: 20130028928Abstract: Increased antigenicity of a membrane bound polypeptide produced from plants is provided by reducing fat content of the plant, plant part, or plant tissue producing the polypeptide. Methods and means of producing such plant material are provided. Methods to produce a protective immune response in animals are provided by administering to the animal the plant, plant part of plant tissue which has reduced fat content and which comprises the polypeptide or by administering to the animal an extracted polypeptide produced from such a plant.Type: ApplicationFiled: July 26, 2012Publication date: January 31, 2013Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: John Howard, Celine Hayden
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Patent number: 8115060Abstract: A method of controlling germination of seed is provided in which a nucleic acid encoding a protein which causes inhibition of germination is transformed into a plant, operably linked with a promoter that expresses during germination. The protein is one in which a restoring substance can be applied to the seed and restores germination. Also provided is an amylase germination preferred promoter.Type: GrantFiled: February 22, 2009Date of Patent: February 14, 2012Assignee: Applied Biotechnology Institute, Inc.Inventors: John Howard, Erin Engelkrout
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Publication number: 20110143398Abstract: Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional ?-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.Type: ApplicationFiled: February 11, 2011Publication date: June 16, 2011Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: John Howard, Gina Fake
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Publication number: 20110097786Abstract: Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional ?-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.Type: ApplicationFiled: October 9, 2010Publication date: April 28, 2011Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: John Howard, Gina Fake
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Publication number: 20110091976Abstract: A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.Type: ApplicationFiled: October 9, 2010Publication date: April 21, 2011Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: Stephen J. Streatfield, Robert Love, Jeff Bray
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Patent number: RE41318Abstract: The invention discloses novel promoter sequences capable of expressing genes in plant cells. The promoters include engineered versions of the maize ubiquitin promoter to increase expression levels beyond those observed with the native ubiquitin promoter and alter the tissue preference. Expression constructs, vectors, transgenic plants and methods are also disclosed.Type: GrantFiled: June 1, 2007Date of Patent: May 4, 2010Assignee: Applied Biotechnology Institute, Inc.Inventors: Joseph M. Jilka, Elizabeth E. Hood, John A. Howard