Abstract: A method for producing a population of T cells that express a chimeric antigen receptor (CAR) comprising the steps of providing a fluid sample including a population of T cells; labeling the population of T cells with magnetic beads; extracting the population of T cells from the fluid sample by flowing the fluid sample through a first conduit that passes through a first magnetic separator device; transducing the population of T cells to express a chimeric antigen receptor (CAR) by contacting the population of T cells with a population of lentivirus in a solution contained in an incubation container; and harvesting the population of T cells by flowing the solution through a second conduit that passes through a second magnetic separator device, wherein the first conduit, the incubation container, and the second conduit are fluidically connected by a network of fluidic lines.

Abstract: A process for magnetically sorting biological objects includes the steps of applying a magnetic field generated by a magnetic assembly to a flexible conduit; flowing a sample fluid containing magnetically labeled biological objects through the flexible conduit to collect the magnetically labeled biological objects on a conduit wall; removing the magnetic field from the flexible conduit; and mechanically deforming the flexible conduit to loosen the magnetically labeled biological objects collected on the conduit wall.

Abstract: A system for aseptically transferring a liquid substance comprises a sterilized vial filled with the liquid substance and sealed with a sterilized septum; a sterilized fluidic assembly including a piercing member including therein first and second channels and having a tip end that operably punctures the sterilized septum, the first and second channels having first and second channel openings at the tip end; a manual air pump; an inlet line fluidically connects the manual air pump to the first channel of the piercing member; and an outlet line fluidically connected to the second channel of the piercing member at one end and hermetically sealed at the other end; and a sterilized pouch enclosing and hermetically sealing the sterilized vial and the piercing member and operably allowing the piercing member to puncture the sterilized septum when the piercing member is manually pushed from the exterior of the sterilized pouch.

Abstract: The current invention relates to the method and apparatus to separate biological entities from a fluid sample. The claimed methods separate biological entities based on size of entities by using acoustic pressure nodes in a microfluidic device. The claimed methods further separate biological entities with magnetic labels and by using a magnetic device. The claimed methods further include combination of microfluidic devices and magnetic devices to separate biological entities from a fluid sample.

Abstract: A microfluidic system for separating biological entities includes a cooling device including a thermoelectric heat pump, a first fan, and a first heat exchanger disposed between the first fan and the thermoelectric heat pump; a first housing structure having a first shell that encases the first fan and the first heat exchanger; a microfluidic device and one or more piezoelectric transducers attached thereto; and a second housing structure reversibly attached to the first housing structure and having a second shell that encloses therein the microfluidic device and the one or more piezoelectric transducers. When the first and second housing structures are coupled, a first air passage is formed between a side of the first heat exchanger and an end of the microfluidic device, a second air passage is formed between the first fan and the piezoelectric transducers, thereby allowing air to circulate between the first and second air passages.

Abstract: The current invention relates to the device and method to separate biological entities from a sample fluid by a microfluidic device. The claimed methods separate biological entities by differentiating the sizes of the biological entities with ultrasound modes. The claimed methods further utilize a multi-staged design that removes smaller size entities at earlier and wider sections and concentrates larger entities at later and narrower sections of a microfluidic channel.

Abstract: The current invention generally relates to apparatus and method to analyze and separate biological entities, including cells, bacteria and molecules from human blood, body tissue, body fluid and other human related biological samples. The claimed apparatus and method analyze, or detect, biological entities based on optical signals received from said entities by using optical detectors. The claimed apparatus and method further separate biological entities with using micro-actuator activated sorting devices.

Abstract: The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation.