Patents Assigned to Applied Imaging
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Publication number: 20100045698Abstract: A method is provided for displaying a client-side multi-component image. A plurality of image component channels derived from captured images of a sample are stored in a server, the plurality of image component channels being stored in the form of a plurality of image data records. A client then requests one or more of the plurality of image data records and the server transmits to the client the one or more of the plurality of image data records requested. At the client, the client-side multi-component image is composited from the image data records transmitted the composited client-side multi-component image is displayed.Type: ApplicationFiled: August 21, 2008Publication date: February 25, 2010Applicant: Applied Imaging CorporationInventors: Kevin Shields, James Raine, Gavin Hope
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Publication number: 20090238435Abstract: The present invention provides methods and systems that acquire digital images of a microscope slide having a large variation in pixel value brightness, like, for instance, fluorescent microscope slides. The methods and the systems generate a well contrasted composite image with preserved areas of low and high fluorescent intensity from the input images.Type: ApplicationFiled: March 21, 2008Publication date: September 24, 2009Applicant: Applied Imaging Corp.Inventor: Kevin Shields
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Publication number: 20090129660Abstract: The present invention provides methods and systems for automatic detection of the location of cell colonies on a specimen slide, in particular under the coverslip of a specimen slide. Slide scanning can be performed using an automated microscope with motorized axes. The location of the colonies can be determined by image analysis, which is followed by automatically finding metaphase cells and associating them with each colony. The invention also provides an automated, Hough-transform-based method for identifying the location of the slide coverslip and, if desired, analyzing only the image area contained within the coverslip.Type: ApplicationFiled: October 16, 2008Publication date: May 21, 2009Applicant: Applied Imaging Corp.Inventor: Mark Gregson
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Patent number: 7233340Abstract: Computerized techniques are provided for linking digitized images of serial sections of a biological tissue sample. Linked, digitized images of the serial section are displayed on a display and manipulation of one digitized image causes other digitized images to be similarly manipulated. To link digitized images, a copy of a digitized image of one serial section is positioned over a digitized image of another serial section. The digitized image of the other serial section is visible through the copy. The copy is registered to the digitized image of the other serial section.Type: GrantFiled: April 30, 2003Date of Patent: June 19, 2007Assignee: Applied Imaging Corp.Inventors: William Hughes, Duncan William Borthwick
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Patent number: 7133543Abstract: Scanning and analysis of cytology and histology samples uses a flatbed scanner to capture images of the structures of interest such as tumor cells in a manner that results in sufficient image resolution to allow for the analysis of such common pathology staining techniques as ICC (immunocytochemistry), IHC (immunohistochemistry) or in situ hybridization. Very large volumes of such material are scanned in order to identify cells or clusters of cells which are positive or warrant more detailed examination, and if analysis at higher resolution is necessary, information regarding these positive events is transferred to a secondary microscope, such as a conventional scanning microscope, to allow further analysis and review of the selected regions of the slide containing the sample.Type: GrantFiled: June 6, 2002Date of Patent: November 7, 2006Assignee: Applied Imaging CorporationInventors: Nico Peter Verwoerd, Johannes Vrolijk, Wilhelmina E. Mesker, Willem C. R. Sloos, Jan Bonnet, Padraig S. O'Kelly, Mark Gregson, Kevin Shields, Hendrikus J. Tanke
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Publication number: 20060051736Abstract: A sample configured to be cut to form a set of serial sections. The sample includes a sample block; at least one tissue sample substantially embedded in the sample block; and a least one control-marker core substantially embedded in the sample block and having a select shape as viewed from an end of the control-marker core, wherein each serial section includes a cross section of the tissue sample and a cross section of the control-marker core, each cross section of the tissue sample is referred to as the tissue section and each cross section of the control-marker core is referred to as the control marker, and wherein each control marker has the select shape.Type: ApplicationFiled: August 11, 2005Publication date: March 9, 2006Applicant: Applied Imaging Corp.Inventors: Kevin Shields, Eric Kanazawa
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Publication number: 20040170309Abstract: Computerized techniques are provided for linking digitized images of serial sections of a biological tissue sample. Linked, digitized images of the serial section are displayed on a display and manipulation of one digitized image causes other digitized images to be similarly manipulated. To link digitized images, a copy of a digitized image of one serial section is positioned over a digitized image of another serial section. The digitized image of the other serial section is visible through the copy. The copy is registered to the digitized image of the other serial section.Type: ApplicationFiled: April 30, 2003Publication date: September 2, 2004Applicant: Applied Imaging Corp.Inventors: William Hughes, Duncan William Borthwick
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Patent number: 6633662Abstract: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity.Type: GrantFiled: July 9, 2001Date of Patent: October 14, 2003Assignee: Applied Imaging CorporationInventor: Ilya Ravkin
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Patent number: 6524798Abstract: The invention provides a high efficiency method for combined immunocytochemistry and in situ hybridization. In one aspect, the method is used to simultaneously determining a cell phenotype and genotype by contacting a cell with an antigen-specific antibody bound to a ligand, contacting the cell with polynucleotide probe to form a complex of the probe and a nucleic acid in the cell, contacting the cell with a detectably labeled anti-ligand, and detecting the polynucleotide-probe complex and the anti-ligand-ligand complex. The presence of the anti-ligand is correlated with the presence of the antigen and the presence of the probe-nucleic acid complex is correlated with the presence of the nucleic acid in the cell.Type: GrantFiled: June 12, 2000Date of Patent: February 25, 2003Assignee: Applied Imaging CorporationInventors: Simon Goldbard, Tsai-Hsia Hong, Michael A. Zoccoli, Emily Lin
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Publication number: 20030012420Abstract: Scanning and analysis of cytology and histology samples uses a flatbed scanner to capture images of the structures of interest such as tumor cells in a manner that results in sufficient image resolution to allow for the analysis of such common pathology staining techniques as ICC (immunocytochemistry), IHC (immunohistochemistry) or in situ hybridization. Very large volumes of such material are scanned in order to identify cells or clusters of cells which are positive or warrant more detailed examination, and if analysis at higher resolution is necessary, information regarding these positive events is transferred to a secondary microscope, such as a conventional scanning microscope, to allow further analysis and review of the selected regions of the slide containing the sample.Type: ApplicationFiled: June 6, 2002Publication date: January 16, 2003Applicant: Applied Imaging CorporationInventors: Nico Peter Verwoerd, Johannes Vrolijk, Wilhelmina E. Mesker, Willem C.R. Sloos, Jan Bonnet, Padraig S. O'Kelly, Mark Gregson, Kevin Shields, Hendrikus J. Tanke
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Publication number: 20020081014Abstract: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity.Type: ApplicationFiled: July 9, 2001Publication date: June 27, 2002Applicant: APPLIED IMAGING CORPORATIONInventor: IIya Ravkin
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Patent number: 6270971Abstract: The invention provides methods and reagents for detecting a chromosomal aberration in an animal chromosome or karyotype. One or more detectably-labeled chromosome-specific probes from a first animal species are hybridized to chromosomes of a second animal species. This results in a banding pattern that can be compared to the pattern found in a normal chromosome or karyotype.Type: GrantFiled: June 30, 1999Date of Patent: August 7, 2001Assignee: Applied Imaging CorporationInventors: Malcolm A Ferguson-Smith, Johannes F Wienberg, Stefan Müller
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Patent number: 6259807Abstract: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multibandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity.Type: GrantFiled: November 19, 1999Date of Patent: July 10, 2001Assignee: Applied Imaging Corp.Inventor: Ilya Ravkin
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Patent number: 6225636Abstract: Techniques for acquiring and analyzing M-FISH images in a manner that allows for proper image registration; and techniques for combining a plurality of registered images to provide labeled images. Epi-illumination embodiments use multiple filter cubes for different subsets of the entire set of desired dyes. The registration problem is addressed by configuring the different multiband cubes so that pairs of cubes have a common dye for which they are configured. Accordingly, a first set of images generated with a first cube includes an image that corresponds to (contains the same features as) one of the images in a second set of images generated with a second cube. This allows the first set of images to be precisely and accurately aligned with the second set of images. A method for generating a labeled image typically includes a normalization of the pixel intensities within each image, followed by a transformation to ratio images.Type: GrantFiled: December 29, 1998Date of Patent: May 1, 2001Assignee: Applied Imaging, Inc.Inventor: Jacques Ginestet
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Patent number: 6169816Abstract: Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multi-bandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity.Type: GrantFiled: May 4, 1998Date of Patent: January 2, 2001Assignee: Applied Imaging, Inc.Inventor: Ilya Ravkin
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Patent number: 6087134Abstract: Methods are provided for analyzing DNA of a rare cell in a cell population. In one embodiment, the method involves covering a cell monolayer with a photosensitive material. By illuminating the area over a cell of interest, the material is solidified, permitting manipulation of the underlying cell and/or protection of the cell from DNA-inactivating agents that destroy DNA in other cells in the monolayer. In another embodiment, the monolayer is overlaid with a solid material that becomes soluble when illuminated. By illuminating the area over a cell of interest, that cell can be specifically exposed and DNA from the cell amplified. The methods are particularly useful for analyzing fetal cells found in maternal blood.Type: GrantFiled: October 23, 1997Date of Patent: July 11, 2000Assignee: Applied Imaging CorporationInventor: Alexander Michael Saunders
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Patent number: 5962234Abstract: An in vitro method of identifying or isolating fetal cells from a blood sample is described. Fetal nucleated erythrocytes or erythroblasts are identified by using an antibody or antibody fragment specific for embryonic hemoglobin or an embryonic hemoglobin chain. Once the fetal cells are identified, they can be treated to render the fetal nucleic acids or proteins available for identification or amplification. Detecting the occurrence or existence of selected fetal nucleic acids or proteins allows a quantitative or qualitative diagnostic or prenatal evaluation, including determining the sex of the fetus, determining chromosomal, single gene or protein abnormalities, and determining the presence or absence of particular genes, nucleic acid sequences or proteins.Type: GrantFiled: September 25, 1998Date of Patent: October 5, 1999Assignee: Applied Imaging CorporationInventor: Mitchell Golbus
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Patent number: 5880473Abstract: Techniques for acquiring and analyzing M-FISH images in a manner that allows for proper image registration; and techniques for combining a plurality of registered images to provide labeled images. Epi-illumination embodiments use multiple filter cubes for different subsets of the entire set of desired dyes. The registration problem is addressed by configuring the different multiband cubes so that pairs of cubes have a common dye for which they are configured. Accordingly, a first set of images generated with a first cube includes an image that corresponds to (contains the same features as) one of the images in a second set of images generated with a second cube. This allows the first set of images to be precisely and accurately aligned with the second set of images. A method for generating a labeled image typically includes a normalization of the pixel intensities within each image, followed by a transformation to ratio images.Type: GrantFiled: July 28, 1997Date of Patent: March 9, 1999Assignee: Applied Imaging, Inc.Inventor: Jacques Ginestet
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Patent number: 5731156Abstract: An in vitro method of identifying or isolating fetal cells from a blood sample is described. Fetal nucleated erythrocytes or erythroblasts are identified by using an antibody or antibody fragment specific for embryonic hemoglobin or an embryonic hemoglobin chain. Once the fetal cells are identified, they can be treated to render the fetal nucleic acids or proteins available for identification or amplification. Detecting the occurrence or existence of selected fetal nucleic acids or proteins allows a quantitative or qualitative diagnostic or prenatal evaluation, including determining the sex of the fetus, determining chromosomal, single gene or protein abnormalities, and determining the presence or absence of particular genes, nucleic acid sequences or proteins.Type: GrantFiled: October 21, 1996Date of Patent: March 24, 1998Assignee: Applied Imaging, Inc.Inventor: Mitchell Golbus
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Patent number: 5489386Abstract: A density gradient medium for the isolation and enrichment of rare cells, including fetal nucleated erythrocytes from a peripheral blood sample is described. The medium comprises a colloidal density gradient medium dispersed in a meltable gel. In one aspect of the invention, the density gradient medium is hypertonic to facilitate separation of maternal red blood cells from fetal blood cells.Type: GrantFiled: January 31, 1994Date of Patent: February 6, 1996Assignee: Applied ImagingInventor: Alexander M. Saunders