Patents Assigned to Applied Nanosystems B.V.
  • Method of purifying a hydrophobin present in a hydrophobin-containing solution
    Patent number: 6903191
    Abstract: The invention relates to a method of purifying a hydrophobin present in a hydrophobin-containing solution. According to the invention the solution is contacted with a surface for the adsorption to that surface, and separated from a solution depleted in hydrophobin. Subsequently the surface is contacted with a solution containing a surfactant at a temperature lower than 90° C. Desorbed hydrophobin is separated from said surface.
    Type: Grant
    Filed: February 2, 2001
    Date of Patent: June 7, 2005
    Assignee: Applied NanoSystems B.V.
    Inventors: Marcel Leo de Vocht, Herman Abel B. Wösten, Joseph Gerard H. Wessels
  • Bacterial ghosts provided with antigens
    Patent number: 6896887
    Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.
    Type: Grant
    Filed: December 13, 2002
    Date of Patent: May 24, 2005
    Assignee: Applied Nanosystems B.V.
    Inventors: Cornelis Johannes Leenhouts, Ranjan Ramasamy, Anton Steen, Jan Kok, Girbe Buist, Oscar Paul Kuipers
  • Export and modification of (poly)peptides in the lantibiotic way
    Patent number: 6861236
    Abstract: The invention includes a method for harvesting a polypeptide produced by a host cell, wherein the polypeptide has not undergone intracellular posttranslational modification, such as dehydration of a serine or a threonine, and/or thioether bridge formation. The invention also includes a method for producing thioether-containing peptides and dehydroalanine/dehydrobutyrine-containing.peptides, wherein thioether rings may be formed extracellularly.
    Type: Grant
    Filed: February 7, 2003
    Date of Patent: March 1, 2005
    Assignee: Applied Nanosystems B.V.
    Inventors: Gert Nikolaas Moll, Cornelis Johannes Leenhouts