Abstract: A method for the regeneration of adsorption material which utilizes the biological activity of a consortium of microorganisms or a single strain of microorganisms. The method utilizes at least one chamber which may contain a variety of materials that adsorb undesirable organic and/or inorganic compounds. The method incorporates a cyclic method of active adsorption of undesirable compounds, sequentially performed with an active biological regeneration cycle. The biological process may have the characteristic of either aerobic, anaerobic, facilitative aerobic, or facilitative anaerobic microorganisms. As a result of the cyclic method of operation, the adsorption cycle maximizes the optimum efficiency of the adsorptive material. The method incorporates the use of a highly active bacterial inoculant that is customized to remove the undesirable-compounds from the adsorptive material.