Patents Assigned to APSE, INC.
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Publication number: 20220145294Abstract: The invention provides methods and compositions for improved production of large quantities of unencapsidated double strand RNA (dsRNA) in vivo. The disclosed methods and compositions, comprising co-expression of genes encoding orotate phosporibosyl transferase, bacteriophage coat protein and dsRNA produce a significant improvement over current in vivo methods of producing unencapsidated dsRNA.Type: ApplicationFiled: July 7, 2020Publication date: May 12, 2022Applicant: APSE, INC.Inventors: John L. KILLMER, Patrick D. McLAUGHLIN, Juan Pedro Humberto ARHANCET
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Publication number: 20210127683Abstract: The invention describes recombinant DNA sequences transcribed into RNA constructs capable of forming Virus Like Particles (VLPs) suitable for insect control applications.Type: ApplicationFiled: October 20, 2020Publication date: May 6, 2021Applicant: APSE, INC.Inventors: John L. Killmer, Juan Pedro Humberto Arhancet, Patrick D. McLaughlin
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Patent number: 10806150Abstract: The invention describes recombinant DNA sequences transcribed into RNA constructs capable of forming Virus Like Particles (VLPs) suitable for insect control applications. Specifically, the disclosure provides a method for controlling target insects comprising, transforming a microbial host with a first DNA sequence comprising a gene encoding a bacteriophage capsid protein and a second DNA sequence encoding an RNA transcript comprising at least one bacteriophage pac sequence coupled to an RNAi precursor sequence, inducing the microbial host to express the first and second DNA sequences, isolating virus-like-particles (VLPs) comprising the capsid protein and RNAi precursor from the microbial host, and contacting the isolated VLPs with the target insects.Type: GrantFiled: December 7, 2016Date of Patent: October 20, 2020Assignee: APSE, INC.Inventors: John L. Killmer, Juan Pedro Humberto Arhancet, Patrick D. McLaughlin
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Patent number: 10704045Abstract: Methods and materials for improved in vivo production of dsRNA are presented. Yields of dsRNA are significantly increased in the presence of capsid protein. The improved yield of dsRNA is not dependent on the presence of specific cognate binding sites for capsid protein associated with the dsRNA, but is dependent on capsid protein.Type: GrantFiled: March 9, 2017Date of Patent: July 7, 2020Assignee: APSE, INC.Inventors: John L. Killmer, Patrick D. McLaughlin, Juan Pedro Humberto Arhancet
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Patent number: 10428329Abstract: Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.Type: GrantFiled: October 17, 2017Date of Patent: October 1, 2019Assignee: APSE, INC.Inventors: Juan Pedro Humberto Arhancet, Juan P. Arhancet, Kimberly Delaney, Kathleen B. Hall, Neena Summers, Edward Oates
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Publication number: 20190093108Abstract: Methods and materials for improved in vivo production of dsRNA are presented. Yields of dsRNA are significantly increased in the presence of capsid protein. The improved yield of dsRNA is not dependent on the presence of specific cognate binding sites for capsid protein associated with the dsRNA, but is dependent on capsid protein.Type: ApplicationFiled: March 9, 2017Publication date: March 28, 2019Applicant: APSE, INC.Inventors: John L. KILLMER, Patrick D. McLAUGHLIN, Juan Pedro Humberto ARHANCET
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Publication number: 20190029265Abstract: The invention describes recombinant sequences and methods for producing RNAs suitable for controlling proliferation of ant species, including Solenopsis, Camponotus, Linepithema, Tapinoma, Tetramorium, and Monomorium spp.Type: ApplicationFiled: January 31, 2017Publication date: January 31, 2019Applicant: APSE, INC.Inventors: John L. KILLMER, Anil KUMAR, Patrick D. McLAUGHLIN, Juan Pedro Humberto ARHANCET
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Publication number: 20180368422Abstract: The invention describes recombinant DNA sequences transcribed into RNA constructs capable of forming Virus Like Particles (VLPs) suitable for insect control applications. Specifically, the disclosure provides a method for controlling target insects comprising, transforming a microbial host with a first DNA sequence comprising a gene encoding a bacteriophage capsid protein and a second DNA sequence encoding an RNA transcript comprising at least one bacteriophage pac sequence coupled to an RNAi precursor sequence, inducing the microbial host to express the first and second DNA sequences, isolating virus-like-particles (VLPs) comprising the capsid protein and RNAi precursor from the microbial host, and contacting the isolated VLPs with the target insects.Type: ApplicationFiled: December 7, 2016Publication date: December 27, 2018Applicant: APSE, INC.Inventors: John L. Killmer, Juan Pedro Humberto Arhancet, Patrick D. McLaughlin
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Publication number: 20180030445Abstract: Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.Type: ApplicationFiled: October 17, 2017Publication date: February 1, 2018Applicant: APSE, INC.Inventors: Juan Pedro Humberto ARHANCET, JUAN P. ARHANCET, Kimberly DELANEY, Kathleen B. HALL, Neena SUMMERS, Edward OATES
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Patent number: 9822361Abstract: Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.Type: GrantFiled: June 5, 2014Date of Patent: November 21, 2017Assignee: APSE, INC.Inventors: Juan Pedro Humberto Arhancet, Juan P. Arhancet, Kimberly Delaney, Kathleen B. Hall, Neena Summers, Edward Oates