Abstract: Systems and methods for detecting deceptive intent of a subject include observing eye movements of the subject and correlating the observed movements to known baseline neurophysiological indicators of deception. A detection system may record eye movement data from the subject, compare the eye movement data to a data model comprising threshold eye movement data samples, and from the comparison make a determination whether or not the subject is lying. The detection system may create an alert if deception is detected. The eye movements detected include saccadic and intersaccadic parameters such as intersaccadic drift velocity. Measurements may be collected in situ with a field testing device, such as a non-invasive, non-contact device attached to the subject's computing device and configured to non-obtrusively record the eye movement data.