Abstract: A method for analyzing hemoglobin by electrophoresis, capable of analyzing hemoglobin A1c (HbA1c) and modified hemoglobin with improved accuracy in a shortened analysis time is provided. The method for analyzing hemoglobin by electrophoresis includes performing electrophoresis under conditions in which an acidic substance having two or more carboxyl groups is present in an electrophoresis solution. At least two of the carboxyl groups of the acidic substance each have an acid dissociation constant (pKa) lower than the pH of the electrophoresis solution at the time of analysis.