Abstract: Provided herein are tubular membrane filter elements, tangential flow filtration systems comprising such filter elements and methods of using such filter elements and filtration systems.
Type:
Grant
Filed:
June 11, 2019
Date of Patent:
April 27, 2021
Assignee:
Artemis Biosystems, Inc.
Inventors:
Maurizio V. Cattaneo, Remco A. Spanjaard
Abstract: Provided herein are tubular membrane filter elements, tangential flow filtration systems comprising such filter elements and methods of using such filter elements and filtration systems.
Type:
Grant
Filed:
April 12, 2017
Date of Patent:
July 23, 2019
Assignee:
Artemis Biosystems, Inc.
Inventors:
Maurizio V. Cattaneo, Remco A. Spanjaard
Abstract: Described herein is a rapid continuous biomanufacturing platform process that combines a perfusion mammalian cell culture with a synchronized purification of the antibodies produced by the cell culture without the use of intermediate media holding tanks or other large retention devices. This method described herein includes continuous cell culture of mammalian cells expressing the biological of interest and comprising a cell retention device wherein the perfusion of fresh media into the reactor and hence the harvest rate of antibody containing spent media from the reactor has a rate of 1 vessel volume per day (vvd) or less obtained by glucose control with a cell density of between 40 million cell/ml and 60 million cell/nil. Immediate recovery and purification of the antibody is obtained by synchronizing the rate of perfusion with the antibody capture and elution step cycle in a column that contains affinity resin that is between 0.01 and 0.001 times the volume of the bioreactor.
Type:
Application
Filed:
January 15, 2016
Publication date:
July 20, 2017
Applicant:
Artemis BioSystems Inc.
Inventors:
MAURIZIO VICTOR CATTANEO, MARK HENRY KAMGA, REMCO ALEXANDER SPANJAARD