Patents Assigned to ARUP Laboratories, Inc.
  • Separating and quantifying unbound target analytes from biological samples
    Patent number: 11969670
    Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.
    Type: Grant
    Filed: July 26, 2021
    Date of Patent: April 30, 2024
    Assignee: ARUP Laboratories, Inc.
    Inventors: Mark M. Kushnir, Alan L. Rockwood
  • Separating and quantifying unbound target analytes from biological samples
    Patent number: 11071927
    Abstract: A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.
    Type: Grant
    Filed: November 15, 2018
    Date of Patent: July 27, 2021
    Assignee: ARUP Laboratories, Inc.
    Inventors: Mark M. Kushnir, Alan L. Rockwood