Abstract: The present invention relates to a method for sampling a bodily fluid from the skin of a test subject by means of a sampling system The method comprises the steps of a) placing the rim of the microneedle assembly in contact with the skin of the test subject, thereby sealing the interior of the housing from the surrounding atmosphere; b) applying a negative penetration pressure by means of the pump to the fluid port and thereby to the interior of the housing, wherein the negative penetration pressure is maintained during a penetration time, whereby the plurality of microneedles penetrates the skin of the test subject; c) lift-off, wherein the plurality of microneedles are removed from the skin during a lift-off time wherein the interior of the housing is at least at the atmospheric pressure; and d) applying negative extraction pressure with the pump during an extraction time whereby the bodily fluid released from the skin flows towards the fluid port and is collected in a sample volume The present invention al

Abstract: Microneedle (100, 200, 300, 720) comprising an elongated body (110, 210, 310) extending along a longitudinal axis from a top end to a bottom end on a substrate (300, 710), where the elongated body comprises an upper portion (120, 220, 320) and a lower portion (130, 230, 330). The lower portion (130, 230, 330) comprises an internal capillary bore hole (260, 730) extending into the substrate (300, 710). The upper portion (120, 220, 320) of the elongated body (110, 210, 310) has a semi-enclosed internal void space (140, 240) formed by at least three body sides whereof two body sides join at a sharp edge and a third body side is provided with an opening slit (150, 250, 350) extending from the lower portion (130, 230, 330) of the elongated body (110, 210, 310) to the upper end of the third body side. The top end of the elongated body (110, 210, 310) is configured as a bevel to create a sharp tip at the top of said edge, said bevel extending to the third body side.

Abstract: A suction applying device (10) for applying suction to a sampling unit (50) for sampling bodily fluid, wherein the suction applying device comprises a syringe body (12) with a hollow interior (14) and a piston (20) movable inside the hollow interior of the syringe body. The syringe body has a rear end (16) through which a piston rod (21) connected to the piston extends outside of the syringe body and by means of which the piston can be moved, and a front end (18) opposite the rear end. The suction applying device further comprises a connection arrangement (130) configured for connection of a sampling unit, wherein the connection arrangement is located at the front end of the syringe body.

Abstract: A microneedle and a chip are disclosed for extraction of fluids. The microneedle (101) provided on a substrate (102), comprises an elongated body (503) extending from a distal end (504) with a bevel (505) to a proximal end (506) on the substrate along a longitudinal axis; the elongated body comprises a capillary bore (507) extending in a longitudinal direction thereof and defines a fluid path (508); the proximal end is integrally connected with the substrate and the capillary bore is in fluid communication with a fluid channel (309) of the substrate. The cross-sectional area of the capillary bore in the distal end is larger than the cross-sectional area of the capillary bore in the proximal end.

Abstract: The present invention relates to a method for analysing susceptibility of a glucose metabolizing microorganism to at least one antibiotic agent, comprising cultivating the microorganism in a culture medium in the presence and absence of antibiotic agent and measuring the rate of change in glucose concentration in the respective media. The invention further relates to a method for assessing efficacy of a candidate antibiotic agent in treatment of a medical condition caused or complicated by an infection by using the method according to the invention, as well as a culture medium suitable for use in the methods.

Abstract: A suction applying device (10) for applying suction to a sampling unit (50) for sampling bodily fluid, wherein the suction applying device comprises a syringe body (12) with a hollow interior (14) and a piston (20) movable inside the hollow interior of the syringe body. The syringe body has a rear end (16) through which a piston rod (21) connected to the piston extends outside of the syringe body and by means of which the piston can be moved, and a front end (18) opposite the rear end. The suction applying device further comprises a connection arrangement (130) configured for connection of a sampling unit, wherein the connection arrangement is located at the front end of the syringe body.

Abstract: A microfabricated sensor (1) for detecting a component in bodily fluid, includes: an inlet (2) for receiving a sample of bodily fluid, a fluid cavity (6) connected to the inlet for receiving the sample of bodily fluid from the inlet, and an RF resonant cavity (13), delimited by walls (14). At least one of the walls forms a separating wall (15), separating the fluid cavity from the RF resonant cavity, wherein the separating wall is configured such that the dielectric properties of the bodily fluid in the fluid cavity provide an influence on the electromagnetic properties of the RF resonant cavity.

Abstract: A microneedle and a chip are disclosed for extraction of fluids. The microneedle (101) provided on a substrate (102), comprises an elongated body (503) extending from a distal end (504) with a bevel (505) to a proximal end (506) on the substrate along a longitudinal axis; the elongated body comprises a capillary bore (507) extending in a longitudinal direction thereof and defines a fluid path (508); the proximal end is integrally connected with the substrate and the capillary bore is in fluid communication with a fluid channel (309) of the substrate. The cross-sectional area of the capillary bore in the distal end is larger than the cross-sectional area of the capillary bore in the proximal end.

Abstract: The disclosure relates to a microfabricated sensor (1) comprising at least one hollow microneedle (2) for minimal invasive extraction of a sample of bodily fluid, a fluid channel (3) connected to the at least one microneedle for receiving a sample of bodily fluid extracted by the at least one microneedle, a microwave transmission line (4) coupled to and extending along at least a portion of the fluid channel, such that the dielectric properties of the fluid in the fluid channel provide an influence on the electrical properties of the transmission line. The disclosure further relates to a method of microfabricating such a sensor and a method of sensing the level of a component in bodily fluid of a patient by providing such a sensor.

Abstract: The disclosure relates to a microfabricated sensor (1) comprising at least one hollow microneedle (2) for minimal invasive extraction of a sample of bodily fluid, a fluid channel (3) connected to the at least one microneedle for receiving a sample of bodily fluid extracted by the at least one microneedle, a microwave transmission line (4) coupled to and extending along at least a portion of the fluid channel, such that the dielectric properties of the fluid in the fluid channel provide an influence on the electrical properties of the transmission line. The disclosure further relates to a method of microfabricating such a sensor and a method of sensing the level of a component in bodily fluid of a patient by providing such a sensor.